Construct: ORF TRCN0000477793
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006537.1_s317c1
- Derived from:
- ccsbBroadEn_09286
- DNA Barcode:
- CCGACTCCTTGTTAGTGGTATGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF30 (90075)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477793
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 90075 | ZNF30 | zinc finger protein 30 | NM_001099437.2 | 99.9% | 99.8% | 1477A>G |
| 2 | human | 90075 | ZNF30 | zinc finger protein 30 | NM_001099438.1 | 99.9% | 99.8% | 1477A>G |
| 3 | human | 90075 | ZNF30 | zinc finger protein 30 | XM_011527443.2 | 99.9% | 99.8% | 1477A>G |
| 4 | human | 90075 | ZNF30 | zinc finger protein 30 | XM_011527444.1 | 99.9% | 99.8% | 1477A>G |
| 5 | human | 90075 | ZNF30 | zinc finger protein 30 | XM_017027423.1 | 99.9% | 99.8% | 1477A>G |
| 6 | human | 90075 | ZNF30 | zinc finger protein 30 | XM_017027424.1 | 99.9% | 99.8% | 1477A>G |
| 7 | human | 90075 | ZNF30 | zinc finger protein 30 | NM_194325.3 | 99.7% | 99.6% | 159_160insGCA;1474A>G |
| 8 | human | 90075 | ZNF30 | zinc finger protein 30 | XM_017027425.1 | 99.7% | 99.6% | 159_160insGCA;1474A>G |
| 9 | human | 90075 | ZNF30 | zinc finger protein 30 | XM_017027426.1 | 99.7% | 99.6% | 159_160insGCA;1474A>G |
| 10 | human | 90075 | ZNF30 | zinc finger protein 30 | XM_017027428.1 | 86.6% | 86.3% | (many diffs) |
| 11 | human | 90075 | ZNF30 | zinc finger protein 30 | NR_024018.2 | 70.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1941
- ORF length:
- 1872
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcccataaa tatgtgggtt tgcagtatca cggatcagtg acatttgagg 121 atgtggccat agccttctcc cagcaggagt gggagagtct ggactcttcc cagaggggct 181 tgtacagaga tgtgatgttg gagaactaca ggaacttggt gtcaatggca ggacattccc 241 gttctaaacc acatgtgatc gccttattgg aacaatggaa agagcctgaa gtgacagtga 301 ggaaagatgg aagaagatgg tgcacagatt tgcagttgga agatgataca atcggctgta 361 aagaaatgcc cacctctgaa aactgtccat cttttgctct acatcagaaa ataagtagac 421 agaaaccacg tgaatgtcag gaatatggaa agaccctttg tcaagactca aagcctgttc 481 aacatgaaag aatacatagt agtgaaaaac ccaacagatg taaagaatgt gggaagaact 541 ttagtaatgg acatcaactc accatacatc agagattgca tgttggtgag aaaccctata 601 aatatgaaaa atgtgggaag gcctttatca gtggctcagc ctttgttaag catgggagaa 661 ttcacactgg tgagaagcca ctcaaatgta agcaatgtgg aaagactatt agtggtagct 721 atcaacttac agtacataag agtattcata ctgggaagaa accatatgag tgcggggaat 781 gtgggaaagc ttttctagta tatggaaagc ttacccggca tcagagtact cacactggtg 841 aaaaaccctt tgggtgtgag gagtgtggga aggccttcag taccttttca tacctggttc 901 aacatcagcg aattcatacc agtgaaaaac cttacgaatg caaagaatgt gggaaggcct 961 ttagcactag ctcacccctt gctaagcatc agagaattca tactggcgag aaaccctatg 1021 aatgtaagga gtgtgggaag tccttcactg tgtatggaca gcttactcga catcagagta 1081 ttcatactgg tgagaaacct tttgaatgta aggaatgtgg aaaggccttt agacttagtt 1141 ccttccttca tgcacatcag cgaattcatg cagagataaa gccctacgga tgcaaggaat 1201 gcgggagaac cttcagtcgt gcctcatatc ttgttcaaca tggaagactt cacactggcg 1261 agaagcccta tgaatgtaag gagtgtggca aggcctttag tactggctca taccttgttc 1321 agcatcagag gatccatact ggggagaaac cctatgaatg taaggaatgt ggcaaagcct 1381 ttattagtcg ccatcagctt accgtacatc aaagggttca tactggagag aaaccctatg 1441 agtgtaagga atgtggcaag gccttcagag tgcacgtaca tctcacacag catcggaaaa 1501 ttcatactga tgtaaagccc tatgaatgta aggaatgtgg aaaggctttt agtcgagccT 1561 CGTACCTTGT ACAACATAGC AGAATCCATA CTGGTAAGAA GCCCTATGAG TGTAAGGAGT 1621 GTGGCAAGGC CTTCAGTTCT GGCTCATACC TTGTTCAGCA TCAAAGAATT CATACTGGGG 1681 AGAAACCCTA TGAATGTAAC AAATGTGGGA AAGCCTTTAC TGTTTATGGA CAACTTATTG 1741 GACATCAGAG TGTTCACACT GGTGAGAAAC CTTTTGAATG TAAGGAATGC GGGAAGGCCT 1801 TTAGACTTAA TTCATTCCTT ACTGAACATC AGCGGGTACA CACTGGTGAG AAACCCTTTA 1861 AATGCAAAAA ATGTGGGAAG ACCTTTAGAT ACAGTTCAGC CCTTAAAGTG CATCTGAGAA 1921 AACATATGAG TGTTATACCC TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1981 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2041 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACCGA CTCCTTGTTA 2101 GTGGTATGTC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt