Construct: ORF TRCN0000477807
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007377.1_s317c1
- Derived from:
- ccsbBroadEn_06171
- DNA Barcode:
- CTCTGATGCGGCGTACGATCGGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STX2 (2054)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477807
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2054 | STX2 | syntaxin 2 | NM_001980.5 | 99.8% | 100% | 861C>A |
| 2 | human | 2054 | STX2 | syntaxin 2 | NM_194356.4 | 95.3% | 93.4% | (many diffs) |
| 3 | human | 2054 | STX2 | syntaxin 2 | NM_001351050.2 | 93.1% | 90% | (many diffs) |
| 4 | human | 2054 | STX2 | syntaxin 2 | NM_001351049.2 | 92.2% | 88.2% | (many diffs) |
| 5 | human | 2054 | STX2 | syntaxin 2 | XM_017018980.2 | 88.5% | 86.6% | (many diffs) |
| 6 | human | 2054 | STX2 | syntaxin 2 | XM_017018983.2 | 85.7% | 83.6% | (many diffs) |
| 7 | human | 2054 | STX2 | syntaxin 2 | XM_017018978.1 | 84.6% | 80.8% | (many diffs) |
| 8 | human | 2054 | STX2 | syntaxin 2 | XM_017018979.2 | 84.6% | 80.8% | (many diffs) |
| 9 | human | 2054 | STX2 | syntaxin 2 | XM_017018981.2 | 83.2% | 76.3% | (many diffs) |
| 10 | human | 2054 | STX2 | syntaxin 2 | XM_017018982.2 | 81.8% | 76.5% | (many diffs) |
| 11 | human | 2054 | STX2 | syntaxin 2 | NM_001351051.2 | 70.4% | 68.6% | (many diffs) |
| 12 | human | 2054 | STX2 | syntaxin 2 | XM_017018984.1 | 66.4% | 62.1% | (many diffs) |
| 13 | human | 2054 | STX2 | syntaxin 2 | XM_017018988.1 | 56.5% | 54.3% | (many diffs) |
| 14 | human | 2054 | STX2 | syntaxin 2 | NM_001351052.2 | 54.9% | 55% | 0_1ins387;474C>A |
| 15 | human | 2054 | STX2 | syntaxin 2 | XM_017018989.1 | 35.5% | 29.7% | (many diffs) |
| 16 | mouse | 13852 | Stx2 | syntaxin 2 | NM_001286033.1 | 84.8% | 89.5% | (many diffs) |
| 17 | mouse | 13852 | Stx2 | syntaxin 2 | NM_001286034.1 | 76.7% | 80.8% | (many diffs) |
| 18 | mouse | 13852 | Stx2 | syntaxin 2 | XM_006504276.2 | 70.3% | 70.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 927
- ORF length:
- 861
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg ggaccggctg ccagacctga cggcgtgtag gaagaatgat gatggagaca 121 cagttgttgt ggttgagaaa gatcatttca tggatgattt cttccatcag gtggaggaga 181 ttagaaacag tattgataaa ataactcaat atgttgaaga agtaaagaaa aaccacagca 241 tcattctttc tgcaccaaac ccggaaggaa aaataaaaga agagcttgaa gatctgaaca 301 aagaaatcaa gaaaactgcg aataaaattc gagccaagtt aaaggctatt gaacaaagtt 361 ttgatcagga tgagagtggg aaccggactt cagtggatct tcggatacga agaacccagc 421 attcggtgct gtctcggaag tttgtggaag ccatggcgga gtacaatgag gcacagactc 481 TGTTTCGGGA GCGGAGCAAA GGCCGCATCC AGCGCCAGCT GGAGATAACT GGGAGAACCA 541 CCACAGACGA CGAGCTAGAA GAGATGCTGG AGAGCGGGAA GCCATCCATC TTCACTTCCG 601 ACATTATATC AGATTCACAA ATTACTAGAC AAGCTCTCAA TGAAATCGAG TCACGTCACA 661 AGGACATCAT GAAGCTGGAG ACCAGCATCC GAGAGTTGCA TGAGATGTTC ATGGACATGG 721 CTATGTTTGT GGAGACTCAG GGTGAAATGA TCAACAACAT AGAAAGAAAT GTTATGAATG 781 CCACAGACTA TGTAGAACAC GCTAAAGAAG AAACAAAAAA AGCTATCAAA TATCAGAGCA 841 AGGCAAGAAG GAAATTGATG TTCATTATTA TTTGTGTAAT TGTTTTGCTT GTGATCCTTG 901 GAATTATCCT AGCAACAACA TTGTCATACC CAACTTTCTT GTACAAAGTG GTTGATATCG 961 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1021 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACTCTGATG 1081 CGGCGTACGA TCGGCCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1141 aagatt