Construct: ORF TRCN0000477808
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011180.1_s317c1
- Derived from:
- ccsbBroadEn_14510
- DNA Barcode:
- AAATATGCGGCTCTGATAAGTTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DNM1P33 (554175)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477808
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 101927579 | DNM1P50 | dynamin 1 pseudogene 50 | NR_145478.1 | 20.9% | (many diffs) | |
| 2 | human | 1759 | DNM1 | dynamin 1 | NM_001005336.3 | 9.9% | 8.5% | (many diffs) |
| 3 | human | 1759 | DNM1 | dynamin 1 | NM_001288737.2 | 9.9% | 8.5% | (many diffs) |
| 4 | human | 1759 | DNM1 | dynamin 1 | NM_001288738.2 | 9.9% | 8.5% | (many diffs) |
| 5 | human | 1759 | DNM1 | dynamin 1 | NM_001288739.2 | 9.7% | 8.4% | (many diffs) |
| 6 | human | 1759 | DNM1 | dynamin 1 | NM_004408.4 | 9.7% | 8.4% | (many diffs) |
| 7 | human | 1759 | DNM1 | dynamin 1 | NM_001374269.1 | 9.7% | 8.3% | (many diffs) |
| 8 | human | 100128285 | DNM1P35 | dynamin 1 pseudogene 35 | NR_024595.3 | 6.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 100
- ORF end:
- 406
- ORF length:
- 306
- Sequence:
-
1 TTCTCAAGCC TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT 61 AGCATCGATT GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCCTTCGTG CTCTCCACAG 121 CAGGCCAGCA AGGCCGAGGA GAATGGTTCC AACAGCTTCA TGCACTCCAT GGTCCCACAG 181 CTGGAGTGGC AGATGGAGAC CACCCAGAGC CTGGTGGACT CCTATGTGGC CATTGTCAAC 241 AAGACCGTGT GGGACCTCAT GGTTGGTCTC ACACCCAAGA CCATCATGCA CCTCATGATC 301 AACAACAACA AGGAGTTTAT CTTCTCGGAG CTGCTGGCCA ACCTGTACTT GCATGGGGAC 361 AAGAACATGC TGATGGAGGA GTCGGCAGAG CAGGCACAGT GGTCATGCCC AACTTTCTTG 421 TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG 481 TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT 541 GGAAAGGACG AAAATATGCG GCTCTGATAA GTTGTACGCG TTAAGTCgac aatcaacctc 601 tggattacaa aatttgtgaa agatt