Construct: ORF TRCN0000477811
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017743.1_s317c1
- Derived from:
- ccsbBroadEn_00512
- DNA Barcode:
- TACTACCATACTGCAAGGTGTTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPO (2056)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477811
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2056 | EPO | erythropoietin | NM_000799.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 648
- ORF length:
- 579
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggggtgcac gaatgtcctg cctggctgtg gcttctcctg tccctgctgt 121 cgctccctct gggcctccca gtcctgggcg ccccaccacg cctcatctgt gacagccgag 181 tcctggagag gtacctcttg gaggccaagg aggccgagaa tatcacgacg ggctgtgctg 241 aacactgcag cttgaatgag aatatcactg tcccagacac caaagttaat ttctatgcct 301 ggaagaggat ggaggtcggg cagcaggccg tagaagtctg gcagggcctg gccctgctgt 361 cggaagctgt cctgcggggC CAGGCCCTGT TGGTCAACTC TTCCCAGCCG TGGGAGCCCC 421 TGCAGCTGCA TGTGGATAAA GCCGTCAGTG GCCTTCGCAG CCTCACCACT CTGCTTCGGG 481 CTCTGGGAGC CCAGAAGGAA GCCATCTCCC CTCCAGATGC GGCCTCAGCT GCTCCACTCC 541 GAACAATCAC TGCTGACACT TTCCGCAAAC TCTTCCGAGT CTACTCCAAT TTCCTCCGGG 601 GAAAGCTGAA GCTGTACACA GGGGAGGCCT GCAGGACAGG GGACAGATTG CCAACTTTCT 661 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 721 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 781 GTGGAAAGGA CGATACTACC ATACTGCAAG GTGTTACACG CGTTAAGTCg acaatcaacc 841 tctggattac aaaatttgtg aaagatt