Construct: ORF TRCN0000477817
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002946.1_s317c1
- Derived from:
- ccsbBroadEn_13867
- DNA Barcode:
- CGAATAAGAGGTGCCGACGAGCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GTF2I (2969)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477817
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2969 | GTF2I | general transcription facto... | NM_001280800.1 | 99.8% | 100% | 520T>C |
| 2 | human | 2969 | GTF2I | general transcription facto... | NM_001518.5 | 28.5% | 28.5% | 520T>C;821A>G;823_2871del |
| 3 | human | 2969 | GTF2I | general transcription facto... | NM_001163636.3 | 28% | 27.9% | (many diffs) |
| 4 | human | 2969 | GTF2I | general transcription facto... | NM_033001.4 | 27.9% | 27.9% | (many diffs) |
| 5 | human | 2969 | GTF2I | general transcription facto... | NM_033000.4 | 27.9% | 27.9% | 520T>C;821A>G;823_2934del |
| 6 | human | 2969 | GTF2I | general transcription facto... | NM_032999.4 | 27.3% | 27.3% | (many diffs) |
| 7 | mouse | 14886 | Gtf2i | general transcription facto... | NM_001080748.2 | 25% | 27.1% | (many diffs) |
| 8 | mouse | 14886 | Gtf2i | general transcription facto... | XM_017320681.1 | 25% | 27.1% | (many diffs) |
| 9 | mouse | 14886 | Gtf2i | general transcription facto... | NM_001080749.2 | 24.9% | 27% | (many diffs) |
| 10 | mouse | 14886 | Gtf2i | general transcription facto... | XM_017320682.1 | 24.7% | 23.9% | (many diffs) |
| 11 | mouse | 14886 | Gtf2i | general transcription facto... | NM_001080747.2 | 24.5% | 26.6% | (many diffs) |
| 12 | mouse | 14886 | Gtf2i | general transcription facto... | XM_006504391.1 | 24.5% | 26.5% | (many diffs) |
| 13 | mouse | 14886 | Gtf2i | general transcription facto... | NM_010365.4 | 24.4% | 26.5% | (many diffs) |
| 14 | mouse | 14886 | Gtf2i | general transcription facto... | XM_017320680.1 | 24.4% | 26.5% | (many diffs) |
| 15 | mouse | 14886 | Gtf2i | general transcription facto... | XM_017320678.1 | 24.4% | 26.5% | (many diffs) |
| 16 | mouse | 14886 | Gtf2i | general transcription facto... | XM_017320679.1 | 24.4% | 26.5% | (many diffs) |
| 17 | mouse | 14886 | Gtf2i | general transcription facto... | XM_017320677.1 | 24.4% | 26.4% | (many diffs) |
| 18 | mouse | 14886 | Gtf2i | general transcription facto... | XM_017320674.1 | 24.3% | 26.4% | (many diffs) |
| 19 | mouse | 14886 | Gtf2i | general transcription facto... | XM_017320675.1 | 24.3% | 26.4% | (many diffs) |
| 20 | mouse | 14886 | Gtf2i | general transcription facto... | XM_017320676.1 | 24.3% | 26.4% | (many diffs) |
| 21 | mouse | 14886 | Gtf2i | general transcription facto... | NM_001080746.2 | 24% | 26% | (many diffs) |
| 22 | mouse | 14886 | Gtf2i | general transcription facto... | XM_006504389.1 | 23.9% | 26% | (many diffs) |
| 23 | mouse | 14886 | Gtf2i | general transcription facto... | XM_017320673.1 | 23.9% | 26% | (many diffs) |
| 24 | mouse | 14886 | Gtf2i | general transcription facto... | XM_006504386.1 | 23.9% | 25.9% | (many diffs) |
| 25 | mouse | 14886 | Gtf2i | general transcription facto... | XM_006504385.1 | 23.8% | 25.9% | (many diffs) |
| 26 | mouse | 14886 | Gtf2i | general transcription facto... | XM_006504384.1 | 23.8% | 25.8% | (many diffs) |
| 27 | mouse | 14886 | Gtf2i | general transcription facto... | XR_001784612.1 | 14.7% | (many diffs) | |
| 28 | mouse | 14886 | Gtf2i | general transcription facto... | XR_001784625.1 | 14.7% | (many diffs) | |
| 29 | mouse | 14886 | Gtf2i | general transcription facto... | XR_001784621.1 | 14.5% | (many diffs) | |
| 30 | mouse | 14886 | Gtf2i | general transcription facto... | XR_001784613.1 | 14.4% | (many diffs) | |
| 31 | mouse | 14886 | Gtf2i | general transcription facto... | XR_001784620.1 | 14.3% | (many diffs) | |
| 32 | mouse | 14886 | Gtf2i | general transcription facto... | XR_001784616.1 | 14.3% | (many diffs) | |
| 33 | mouse | 14886 | Gtf2i | general transcription facto... | XR_001784614.1 | 14.2% | (many diffs) | |
| 34 | mouse | 14886 | Gtf2i | general transcription facto... | XR_001784607.1 | 14.2% | (many diffs) | |
| 35 | mouse | 14886 | Gtf2i | general transcription facto... | XR_001784611.1 | 13.7% | (many diffs) | |
| 36 | mouse | 14886 | Gtf2i | general transcription facto... | XR_001784610.1 | 13.7% | (many diffs) | |
| 37 | mouse | 14886 | Gtf2i | general transcription facto... | XR_001784619.1 | 12.7% | (many diffs) | |
| 38 | mouse | 14886 | Gtf2i | general transcription facto... | XR_001784609.1 | 12.5% | (many diffs) | |
| 39 | mouse | 14886 | Gtf2i | general transcription facto... | XR_001784622.1 | 11.8% | (many diffs) | |
| 40 | mouse | 14886 | Gtf2i | general transcription facto... | XR_001784615.1 | 11.5% | (many diffs) | |
| 41 | mouse | 14886 | Gtf2i | general transcription facto... | XR_001784617.1 | 11.3% | (many diffs) | |
| 42 | mouse | 14886 | Gtf2i | general transcription facto... | XR_001784618.1 | 11.3% | (many diffs) | |
| 43 | mouse | 14886 | Gtf2i | general transcription facto... | XR_001784608.1 | 11.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 888
- ORF length:
- 822
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccaagttgca atgtccaccc tccccgttga agatgaggag tcctcggaga 121 gcaggatggt ggtgacattc ctcatgtcag ctctcgagtc catgtgtaaa gaactggcca 181 agtccaaagc cgaagtggcc tgcattgcag tgtatgaaac agacgtgttt gtcgtcggaa 241 ctgaaagagg acgtgctttt gtcaatacca gaaaggattt tcaaaaagat tttgtaaaat 301 attgtgttga agaagaagaa aaagctgcag agatgcataa aatgaaatct acaacccagg 361 caaatcggat gagtgtagat gctgtagaaa ttgaaacact cagaaaaaca gttgaggact 421 atttctgctt ttgctatGGG AAAGCTTTAG GCAAATCCAC AGTGGTACCT GTACCATATG 481 AGAAGATGCT GCGAGACCAG TCGGCTGTGG TAGTGCAGGG GCTTCCGGAA GGTGTTGCCT 541 TTAAACACCC CGAGAACTAT GATCTTGCAA CCCTGAAATG GATTCTGGAG AACAAAGCAG 601 GGATTTCATT CATCATTAAG AGACCTTTTT TAGAGCCAAA GAAGCATGTA GGTGGTCGTG 661 TGATGGTAAC AGATGCTGAC AGGTCAATAC TATCTCCAGG TGGAAGTTGT GGCCCCATCA 721 AAGTGAAAAC TGAACCCACA GAAGATTCTG GCATTTCCCT GGAAATGGCA GCTGTGACAG 781 TAAAGGAAGA ATCAGAAGAT CCTGATTATT ATCAATATAA CATTCAAGGA AGCCACCATT 841 CTTCAGAGGG CAATGAAGGC ACAGAAATGG AAGTACCAGC AGAAGGTTAC CCAACTTTCT 901 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 961 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1021 GTGGAAAGGA CGACGAATAA GAGGTGCCGA CGAGCCAACG CGTTAAGTCg acaatcaacc 1081 tctggattac aaaatttgtg aaagatt