Transcript: Mouse NM_001080749.2

Mus musculus general transcription factor II I (Gtf2i), transcript variant 5, mRNA.

Source:
NCBI, updated 2017-06-03
Taxon:
Mus musculus (mouse)
Gene:
Gtf2i (14886)
Length:
4349
CDS:
384..3272

Additional Resources:

NCBI RefSeq record:
NM_001080749.2
NBCI Gene record:
Gtf2i (14886)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001080749.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000304427 ACTCATCACCCAACGTTAATA pLKO_005 2761 CDS 100% 15.000 21.000 N Gtf2i n/a
2 TRCN0000086101 GACCCTGATTACTATCAGTAT pLKO.1 1116 CDS 100% 0.495 0.693 N Gtf2i n/a
3 TRCN0000316034 GACCCTGATTACTATCAGTAT pLKO_005 1116 CDS 100% 0.495 0.693 N Gtf2i n/a
4 TRCN0000304428 CCAAGTGGGCAATCGAATTAA pLKO_005 1847 CDS 100% 15.000 12.000 N Gtf2i n/a
5 TRCN0000304426 CCTCGCCTTGAGAGGATATTG pLKO_005 1512 CDS 100% 13.200 10.560 N Gtf2i n/a
6 TRCN0000086099 CCCAGTAACAGAAGAAATTAA pLKO.1 3200 CDS 100% 15.000 10.500 N Gtf2i n/a
7 TRCN0000364550 ATGGGAAAGCTTTAGGCAAAT pLKO_005 754 CDS 100% 10.800 7.560 N GTF2I n/a
8 TRCN0000086098 CCTGTGCTAGTCAGTGCTTTA pLKO.1 3647 3UTR 100% 10.800 7.560 N Gtf2i n/a
9 TRCN0000315959 CCTGTGCTAGTCAGTGCTTTA pLKO_005 3647 3UTR 100% 10.800 7.560 N Gtf2i n/a
10 TRCN0000086100 CCCAATGATCTCTATGTGGAA pLKO.1 1758 CDS 100% 2.640 1.848 N Gtf2i n/a
11 TRCN0000222122 CCACAGAAGATTCTGGCATTT pLKO.1 1054 CDS 100% 10.800 6.480 N GTF2I n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001080749.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000477817 CGAATAAGAGGTGCCGACGAGCCA pLX_317 54.7% 24.9% 27% V5 (many diffs) n/a
2 ccsbBroadEn_13867 pDONR223 100% 24.9% 26.9% None (many diffs) n/a
3 ccsbBroad304_13867 pLX_304 0% 24.9% 26.9% V5 (many diffs) n/a
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