Construct: ORF TRCN0000477824
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003055.1_s317c1
- Derived from:
- ccsbBroadEn_05145
- DNA Barcode:
- TGCTAACATTACCCTGGCCTGTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BHLHA15 (168620)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477824
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 168620 | BHLHA15 | basic helix-loop-helix fami... | NM_177455.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 636
- ORF length:
- 567
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagaccaag aaccggcccc cacggcgccg ggccccggtg caggacacag 121 aggccacccc cggggagggg acgcccgacg ggtccctgcc gaacccgggg ccagagccgg 181 ccaagggtct gcggagccgg ccggcccggg ccgcagcaag ggctccgggc gagggcaggc 241 gcaggcggcc aggaccctcc gggcccggtg gccGTCGTGA CAGCAGCATC CAGCGGCGGC 301 TGGAGAGCAA CGAGAGGGAG CGGCAGCGGA TGCACAAGCT AAATAACGCC TTCCAGGCCC 361 TGCGTGAAGT CATCCCCCAC GTGCGCGCGG ACAAGAAGCT CTCCAAGATC GAGACGCTCA 421 CGCTGGCCAA GAACTACATC AAATCGCTGA CGGCCACCAT CCTGACCATG TCCAGCAGCC 481 GCCTCCCAGG CCTGGAGGGG CCGGGCCCCA AGCTCTACCA GCACTACCAG CAGCAGCAGC 541 AGGTGGCTGG GGGTGCGTTG GGGGCCACGG AGGCCCAGCC CCAGGGCCAC CTGCAGAGGT 601 ACTCCACGCA GATCCACAGC TTCCGAGAGG GCACCTTGCC AACTTTCTTG TACAAAGTGG 661 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 721 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 781 ATGCTAACAT TACCCTGGCC TGTCCACGCG TTAAGTCgac aatcaacctc tggattacaa 841 aatttgtgaa agatt