Construct: ORF TRCN0000477830
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016824.1_s317c1
- Derived from:
- ccsbBroadEn_14774
- DNA Barcode:
- GAACCACCCACTGAAGCTATACAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- POU3F2 (5454)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477830
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5454 | POU3F2 | POU class 3 homeobox 2 | NM_005604.4 | 98.9% | 20.6% | (many diffs) |
| 2 | mouse | 18992 | Pou3f2 | POU domain, class 3, transc... | NM_008899.2 | 93% | 20.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 537
- ORF length:
- 471
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gaccgcagcg tctaaccact acagcctgct cacctccagc gcctccatcg 121 tgcacgccga gccgcccggc ggcatgcagc agggcgcggg gggctaccgc gaagcgcaga 181 gcctggtgca gggcgactac ggcgctctgc agagcaacgg acacccgctc agccacgctc 241 accagtggat cacagcgctg tcccaaccgc cgcggcggcg ggggcggtgg cggcggcggg 301 gggggcgggg gcggcggcgg gggcggcggc gacggctccc cgtggtccac cagccccctg 361 ggccagccgg acatcaagcc ctcggtggtg gtgcagcagg gcggccgcgg agacgagctg 421 cacgggccag gcgccctgca gcagcagcat cagcagcagc aacagcagca gcaacagcag 481 caacagcagc agcagcagca gcaacagcgg ccgccgcatc tggtgcacca cgccgctaac 541 caccacccgg gacccggggc atggcggagc gcggcggctg cagcgcacct cccaccctcc 601 atgggagcgt ccaacggcgg cttgctctac tcgcagccca gcttcacggt gaacggcatg 661 ctgggcgccg gcgggcagtc ggccgggctg caccaccacg gcctgcggga cgcgcacgac 721 gagccacacc atgccgacca ccacccgcac ccgcactcgc acccacacca gcagccgccg 781 cccccgccgc ccccgcaggg tccgcctggc cacccaggcg cgcaccacga cccgcactcg 841 gacgaggaca cgccgacctc ggacgacctg gagcagttcg ccaagcagtt caagcagcgg 901 cggatcaaac tgggatttac acaagcggac gtggggctgg ctctgggcac cctgtatggc 961 aacgtgttct cgcagaccac catctgcagg tttgaggccc tgcagctgag cttcaagaac 1021 atgtgcaagc tgaagccttt gttgaacaag tggttggagg aggcggactc gtcctcgggc 1081 agccccacga gcatagacaa gatcgcagcg caagggcgca agcggaaaaa gcggacctcc 1141 atcGAGGTGA GCGTCAAGGG GGCTCTGGAG AGCCATTTCC TCAAATGCCC CAAGCCCTCG 1201 GCCCAGGAGA TCACCTCCCT CGCGGACAGC TTACAGCTGG AGAAGGAGGT GGTGAGAGTT 1261 TGGTTTTGTA ACAGGAGACA GAAAGAGAAA AGGATGACCC CTCCCGGAGG GACTCTGCCG 1321 GGCGCCGAGG ATGTGTACGG GGGGAGTAGG GACACTCCAC CACACCACGG GGTGCAGACG 1381 CTCGTCCAGT GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC 1441 CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG 1501 ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGAACC ACCCACTGAA GCTATACATA 1561 CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt