Transcript: Mouse NM_008899.2

Mus musculus POU domain, class 3, transcription factor 2 (Pou3f2), mRNA.

Source:
NCBI, updated 2017-06-10
Taxon:
Mus musculus (mouse)
Gene:
Pou3f2 (18992)
Length:
6272
CDS:
236..1573

Additional Resources:

NCBI RefSeq record:
NM_008899.2
NBCI Gene record:
Pou3f2 (18992)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_008899.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000075428 AGGCGGATCAAACTCGGATTT pLKO.1 1079 CDS 100% 10.800 8.640 N Pou3f2 n/a
2 TRCN0000230048 GACTTATAATGGGCATATTTA pLKO_005 3537 3UTR 100% 15.000 10.500 N POU3F2 n/a
3 TRCN0000075431 CGGATCAAACTCGGATTTACT pLKO.1 1082 CDS 100% 5.625 3.938 N Pou3f2 n/a
4 TRCN0000075429 GCTGAAGCCTTTGTTGAACAA pLKO.1 1210 CDS 100% 4.950 3.465 N Pou3f2 n/a
5 TRCN0000417373 GCTGCAGCAACAGCATCAACA pLKO_005 604 CDS 100% 4.950 3.465 N Pou3f2 n/a
6 TRCN0000218221 TGAAGCCTTTGTTGAACAAGT pLKO_005 1212 CDS 100% 4.950 3.465 N POU3F2 n/a
7 TRCN0000222136 GCCTTTGTTGAACAAGTGGTT pLKO.1 1216 CDS 100% 2.640 1.848 N POU3F2 n/a
8 TRCN0000075430 GCAGCCGAGCTTCACGGTGAA pLKO.1 814 CDS 100% 0.000 0.000 N Pou3f2 n/a
9 TRCN0000075432 GCCGGACATCAAGCCCTCGGT pLKO.1 535 CDS 100% 0.000 0.000 N Pou3f2 n/a
10 TRCN0000230046 TGGAGAGCCATTTCCTCAAAT pLKO_005 1347 CDS 100% 13.200 7.920 N POU3F2 n/a
11 TRCN0000415106 TGGAGAGCCATTTCCTCAAAT pLKO_005 1347 CDS 100% 13.200 7.920 N Pou3f2 n/a
12 TRCN0000419405 GAGCAGTTCGCCAAGCAATTC pLKO_005 1052 CDS 100% 10.800 6.480 N Pou3f2 n/a
13 TRCN0000222138 GAGGTGGTGAGAGTTTGGTTT pLKO.1 1427 CDS 100% 4.950 2.970 N POU3F2 n/a
14 TRCN0000230047 AGAAGGAGGTGGTGAGAGTTT pLKO_005 1422 CDS 100% 4.950 2.475 Y POU3F2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_008899.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14774 pDONR223 93.3% 93% 20.5% None (many diffs) n/a
2 ccsbBroad304_14774 pLX_304 0% 93% 20.5% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000477830 GAACCACCCACTGAAGCTATACAT pLX_317 19.1% 93% 20.5% V5 (not translated due to prior stop codon) (many diffs) n/a
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