Construct: ORF TRCN0000477834
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000021.1_s317c1
- Derived from:
- ccsbBroadEn_05309
- DNA Barcode:
- GTTTACCTGATGACCTTCCGGGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OR52B2 (255725)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477834
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 255725 | OR52B2 | olfactory receptor family 5... | NM_001004052.1 | 100% | 100% | |
| 2 | mouse | 259063 | Olfr691 | olfactory receptor 691 | NM_147061.1 | 84.2% | 87.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1038
- ORF length:
- 969
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagtcacacc aatgttacca tcttccatcc tgcagttttt gtccttcctg 121 gcatccctgg gttggaggct tatcacattt ggctgtcaat acctctttgc ctcatttaca 181 tcactgcagt cctgggaaac agcatcctga tagtggttat tgtcatggaa cgtaaccttc 241 atgtgcccat gtatttcttc ctctcaatgc tggccgtcat ggacatcctg ctgtctacca 301 ccactgtgcc caaggcccta gccatctttt ggcttcaagc acataacatt gcttttgatg 361 cctgtgtcac ccaaggcttc tttgtccata tgatgtttgt gggggagtca gctatcctgt 421 tagccatggc ctttgatcgc tttgtggcca tttgtgcccc actgagatat acaacagtgc 481 taacatggcc tgttgtgggg aggattgctc tggccgtcat cacccgaagc ttctgcatca 541 tcttcccagt catattcttg ctgaagcggc tgcccttctg cctaaccaac attgttcctc 601 actcctactg tgagcatatt ggagtggctc gtttagcctg tgctgacatc actgttaaca 661 tttggtatgg cttctcagtg cccattgtca tggtcatctt ggatgttatc ctcatcgctg 721 tgtcttactc actgatccTC CGAGCAGTGT TTCGTTTGCC CTCCCAGGAT GCTCGGCACA 781 AGGCCCTCAG CACTTGTGGC TCCCACCTCT GTGTCATCCT TATGTTTTAT GTTCCATCCT 841 TCTTTACCTT ATTGACCCAT CATTTTGGGC GTAATATTCC TCAACATGTC CATATCTTGC 901 TGGCCAATCT TTATGTGGCA GTGCCACCAA TGCTGAACCC CATTGTCTAT GGTGTGAAGA 961 CTAAGCAGAT ACGTGAGGGT GTAGCCCACC GGTTCTTTGA CATCAAGACT TGGTGCTGTA 1021 CCTCCCCTCT GGGCTCATTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1081 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1141 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGTTTACC TGATGACCTT 1201 CCGGGGAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt