Construct: ORF TRCN0000477857
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003144.1_s317c1
- Derived from:
- ccsbBroadEn_13274
- DNA Barcode:
- TCCGGTGGTTTGAGCGACAGGCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCNYL1 (151195)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477857
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 151195 | CCNYL1 | cyclin Y like 1 | NM_152523.2 | 82.3% | 79.2% | 121_221del;258_309del |
| 2 | human | 151195 | CCNYL1 | cyclin Y like 1 | NM_001142300.1 | 77.2% | 77.2% | 1_210del |
| 3 | human | 151195 | CCNYL1 | cyclin Y like 1 | NM_001330218.1 | 66.2% | 63.7% | 1_210del;331_431del;468_519del |
| 4 | human | 641367 | LOC641367 | cyclin Y like 1 pseudogene | NR_024523.1 | 25.1% | (many diffs) | |
| 5 | mouse | 227210 | Ccnyl1 | cyclin Y-like 1 | NM_001097644.1 | 77% | 78% | (many diffs) |
| 6 | mouse | 227210 | Ccnyl1 | cyclin Y-like 1 | XM_006495953.3 | 71.6% | 75.3% | (many diffs) |
| 7 | mouse | 227210 | Ccnyl1 | cyclin Y-like 1 | XM_011238499.2 | 52.9% | 53.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 783
- ORF length:
- 714
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcccgaagat ttagctttgg agtcaaaccc ttctgaccat ccaagggcaa 121 gcacaatttt cctgagcaaa tctcaaacgg atgtgcgaga aaagaggaag agcaaccatt 181 tgaaccattg tgaccttagc aatatattac cacataaaga acagcgagaa aaagttccag 241 aggaatactt taagcatgat cctgagcaca aatttattta cagatttgtt cgtactcttt 301 ttagtgctgc acagctaaca gctgaatgtg caatagtaac tttggtttac ttagaaaggc 361 ttttAACTTA TGCTGAAATC GACATTTGTC CCACCAACTG GAAAAGGATT GTTCTGGGAG 421 CCATTCTTCT TGCCTCCAAG GTTTGGGACG ATCAGGCTGT ATGGAATGTG GACTACTGCC 481 AGATCCTCAA GGACATTACA GTTGAGGACA TGAATGAAAT GGAAAGGCAT TTTCTGGAGC 541 TTCTTCAGTT TAATATTAAT GTTCCTGCCA GTGTTTATGC CAAATACTAC TTTGACCTTC 601 GCTCCTTAGC AGATGACAAC AACCTGAATT TTCTATTTGC TCCTCTTAGC AAAGAAAGAG 661 CACAGAACCT AGAGGCTATT TCTAGATTGT GTGAAGACAA AGACTTGTGT AGAGCCGCTA 721 TGAGAAGGTC TTTCAGTGCT GATAACTTCA TTGGTATTCA GCGCTCTAAA GCCATCCTCT 781 CTTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 841 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 901 GGCTTTATAT ATCTTGTGGA AAGGACGATC CGGTGGTTTG AGCGACAGGC CAACGCGTTA 961 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt