Construct: ORF TRCN0000477860
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004254.1_s317c1
- Derived from:
- ccsbBroadEn_02341
- DNA Barcode:
- CAGTTCTTACCAGCCGAGTAATGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CNIH1 (10175)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477860
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10175 | CNIH1 | cornichon family AMPA recep... | NM_005776.3 | 100% | 100% | |
| 2 | mouse | 12793 | Cnih1 | cornichon family AMPA recep... | NM_009919.2 | 91.8% | 100% | (many diffs) |
| 3 | mouse | 12793 | Cnih1 | cornichon family AMPA recep... | XM_006518505.1 | 54.9% | 59.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 501
- ORF length:
- 432
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgttcacg ttcgcggcct tctgctacat gctggcgctg ctgctcactg 121 ccgcgctcat cttcttcgcc atttggcaca ttatagcatt tgatgagctg aagactgatt 181 acaagaatcc tatagaccag tgtaataccc tgaatcccct tgtactccca gagtacctca 241 tccacgcttt cttcTGTGTC ATGTTTCTTT GTGCAGCAGA GTGGCTTACA CTGGGTCTCA 301 ATATGCCCCT CTTGGCATAT CATATTTGGA GGTATATGAG TAGACCAGTG ATGAGTGGCC 361 CAGGACTCTA TGACCCTACA ACCATCATGA ATGCAGATAT TCTAGCATAT TGTCAGAAGG 421 AAGGATGGTG CAAATTAGCT TTTTATCTTC TAGCATTTTT TTACTACCTA TATGGCATGA 481 TCTATGTTTT GGTGAGCTCT TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 541 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 601 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACAGT TCTTACCAGC 661 CGAGTAATGA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt