Construct: ORF TRCN0000477865
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013907.1_s317c1
- Derived from:
- ccsbBroadEn_14405
- DNA Barcode:
- CCCGTACCTTAAGAACAATTAAAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- EFHB (151651)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477865
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 151651 | EFHB | EF-hand domain family member B | NM_001330688.2 | 93.2% | 90.9% | (many diffs) |
| 2 | human | 151651 | EFHB | EF-hand domain family member B | XM_011533385.2 | 92.7% | 91.9% | (many diffs) |
| 3 | human | 151651 | EFHB | EF-hand domain family member B | NM_144715.4 | 80.1% | 79.4% | (many diffs) |
| 4 | human | 151651 | EFHB | EF-hand domain family member B | XM_011533383.3 | 79.3% | 77.4% | (many diffs) |
| 5 | human | 151651 | EFHB | EF-hand domain family member B | XM_011533382.2 | 77.6% | 76.9% | (many diffs) |
| 6 | human | 151651 | EFHB | EF-hand domain family member B | XM_005264889.2 | 77.4% | 64.3% | (many diffs) |
| 7 | human | 151651 | EFHB | EF-hand domain family member B | XR_001740031.2 | 68.6% | (many diffs) | |
| 8 | human | 151651 | EFHB | EF-hand domain family member B | XM_017005743.2 | 67.9% | 67.1% | 0_1ins642;11C>T;1353T>A |
| 9 | human | 151651 | EFHB | EF-hand domain family member B | XM_017005744.2 | 67.9% | 67.1% | 0_1ins642;11C>T;1353T>A |
| 10 | human | 151651 | EFHB | EF-hand domain family member B | XM_017005745.2 | 64.5% | 48.9% | (many diffs) |
| 11 | human | 151651 | EFHB | EF-hand domain family member B | XR_940383.2 | 58.2% | (many diffs) | |
| 12 | human | 151651 | EFHB | EF-hand domain family member B | XM_017005742.2 | 36.9% | 37% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2058
- ORF length:
- 1992
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga accaagacag gaaatggaaa aagagtctac ctgtgtttta atgaaaccca 121 acacagagat taagcttcct gtggaggtgg acattggact aacccaagcc gaggggccag 181 atgagactaa gaatacagag ccccaaatgg gcttggtgat agaacctccc caatgccagt 241 ttgcccaaca acatgaacag agaaaggagg ctggaaacat tgaatcagga gtggaacctc 301 cagatcgcat cagacccata tactctggga agttttttga tcggacccct tgctggccaa 361 gtgcaggaaa agttattcca gttggttaca gagttgcaac ctgcttgact gaaaaacttc 421 ccaggctaat tactccacct gaagcaaaaa agtatttcaa cttcagatat ccacctgctg 481 gagtagaaag agtattttac ggaagagcaa atgatcccca gattgcacct tatttgacac 541 atggaattag atctaaaatt tcgatactgg caaacacatt gataaaccca cagcctatta 601 ccacatttca acagaaaatt aaagataaaa aagaatctat atatcttagc aatcgacgag 661 caccattagg aaaatctcac gatcaagcac caggattacc aaaaggcatg gacacaatca 721 atacgacatt tgggacagca gtcatcaaag aatactctgc taaagatgtg gtgaatccac 781 caaaatccta tgaagaagta tttaaagaag gaaatgaagg acatgatttg tatgttgttt 841 ctcacaatga ttattatgca ggagaggcaa agaaccgaaa gtataaccca tcaagtttcc 901 ataggtgtag tgtgtatgga gtaccaacac cacattttaa tgatggacga gccatggcaa 961 aatctctata ttggctccat gaactacaaa tgaaaagagg agctaagttt gtatccaaaa 1021 gagcagatga tttcaaagaa aagtttcaac ataaacttgg aagagtttta gatcccattg 1081 cagaaacaat gaatgttccc ccagactgca catttggagc ttgtctccgt cctgaggaat 1141 atggagttgg tgatctcatc cataatagac ttccggatga atatcttcga ggcaaggata 1201 gacagcgagc cctgattgca gcagttcggc atcacctgaa gaaagttaat taccaaaagt 1261 ttgacacttt gctggcagcc ttcaggcact atgacaagaa gggagatggg atgatagata 1321 aagacgagct gcaggaagct tgtgaccagg ccaacttgag tttagatgac aagctcctgg 1381 accagctatt tgactactgt gatgtggata atgatggctt cattaactat ctggaattcg 1441 caaattttct taactggaaa gacaaaatgc ttcttaaaga gtatgaagag agggtcatta 1501 ttaaaggtag aaaaccagat tgtgtaaacc ctactgaggc taatgttgaa gaacctgaac 1561 aaactctcct cataaagcca gaagatattg tcttaaaaga agcaggaagc acagaaaaga 1621 ctcTCCGGAC ACTTCTGAGA CCAAGTGATA AAGTTTCCAA CTACTATAAG ACAACTTCTT 1681 CTGAGATCAA TGCAATTGTA GGAGCCATTC CTTCTACTTG TTACCCCATT TGTGGTGTTC 1741 CAACCATTCG ATCTGACATT CCTGCTCCCC GAATTCGTCG CATCAGTGAC AGAACTAATT 1801 ATGGTGAAGA AGGTAGTGCA TATTCACTAC TATATCCTAC CATTTTTGCC CGGAAAGGAG 1861 TGTTTGAAAG AGACTTCTTC AAGACCAGAT CAAAAGAAGA GATTGCAGAG ATATTGTGTA 1921 ACATTGGTGT CAAACTGTCT GATGAAGAAT TTGAAAATGT ATGGAATCTT GCATCAAAAA 1981 AGCATCACAG AGGAGAAGTT TGTGTTGAGA ACATCAGAAA TGTTCTAGAT GAGCTACGGC 2041 ATGCAGACCG GATCAAGTGA AAAACACTCA TGTGCCCAAC TTTCTTGTAC AAAGTGGTTG 2101 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2161 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACC 2221 CGTACCTTAA GAACAATTAA ACACGCGTTA AGTCgacaat caacctctgg attacaaaat 2281 ttgtgaaaga tt