Transcript: Human XM_011533385.2

PREDICTED: Homo sapiens EF-hand domain family member B (EFHB), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
EFHB (151651)
Length:
2233
CDS:
206..2074

Additional Resources:

NCBI RefSeq record:
XM_011533385.2
NBCI Gene record:
EFHB (151651)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011533385.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000417732 GACACAACCAATACGACATTT pLKO_005 710 CDS 100% 13.200 18.480 N EFHB n/a
2 TRCN0000055582 GCAGACCGGATCAAGTGTAAA pLKO.1 2042 CDS 100% 13.200 18.480 N EFHB n/a
3 TRCN0000055579 CCACAGCCTATTACCACATTT pLKO.1 587 CDS 100% 13.200 10.560 N EFHB n/a
4 TRCN0000434280 CAACTTCTTCTGAGATCAATG pLKO_005 1671 CDS 100% 10.800 8.640 N EFHB n/a
5 TRCN0000413229 AGTGCATATTCACTACTATAT pLKO_005 1814 CDS 100% 13.200 9.240 N EFHB n/a
6 TRCN0000055580 CGCATCAGTGACAGAACTAAT pLKO.1 1778 CDS 100% 13.200 9.240 N EFHB n/a
7 TRCN0000055578 GCCAGAAGATATTGTCTTAAA pLKO.1 1576 CDS 100% 13.200 9.240 N EFHB n/a
8 TRCN0000415882 GTTGGTGATCTCATCCATAAT pLKO_005 1145 CDS 100% 13.200 9.240 N EFHB n/a
9 TRCN0000055581 CCCAACAACATGAACAGAGAA pLKO.1 243 CDS 100% 4.950 3.465 N EFHB n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011533385.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14405 pDONR223 100% 92.7% 91.9% None (many diffs) n/a
2 ccsbBroad304_14405 pLX_304 0% 92.7% 91.9% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000477865 CCCGTACCTTAAGAACAATTAAAC pLX_317 22.6% 92.7% 91.9% V5 (not translated due to prior stop codon) (many diffs) n/a
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