Construct: ORF TRCN0000477871
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001947.1_s317c1
- Derived from:
- ccsbBroadEn_09270
- DNA Barcode:
- TGTAACACCCCAATTATTCCGGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PNPT1 (87178)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477871
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 87178 | PNPT1 | polyribonucleotide nucleoti... | NM_033109.5 | 99.9% | 99.8% | 361A>G;1390C>A |
| 2 | human | 87178 | PNPT1 | polyribonucleotide nucleoti... | XM_005264629.2 | 89.6% | 89.6% | 0_1ins240;121A>G;1150C>A |
| 3 | human | 87178 | PNPT1 | polyribonucleotide nucleoti... | XM_017005172.1 | 89.6% | 89.6% | 0_1ins240;121A>G;1150C>A |
| 4 | human | 87178 | PNPT1 | polyribonucleotide nucleoti... | XR_001739010.1 | 75% | (many diffs) | |
| 5 | mouse | 71701 | Pnpt1 | polyribonucleotide nucleoti... | NM_027869.1 | 87.1% | 90.8% | (many diffs) |
| 6 | mouse | 71701 | Pnpt1 | polyribonucleotide nucleoti... | XM_011243751.2 | 87.1% | 90.8% | (many diffs) |
| 7 | mouse | 71701 | Pnpt1 | polyribonucleotide nucleoti... | XM_006514813.3 | 55.9% | 58.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2415
- ORF length:
- 2349
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcctgcagg tactgctgct cgtgcctccg gctccggccc ctgagcgatg 121 gtcctttcct tctgccacgg cgggatcggg cactcaccca gttgcaagtg cgagcactat 181 ggagtagcgc agggtctcga gctgtggccg tggacttagg caacaggaaa ttagaaatat 241 cttctggaaa gctggccaga tttgcagatg gctctgctgt agtacagtca ggtgacactg 301 cagtaatggt cacagcggtc agtaaaacaa aaccttcccc ttcccagttt atgcctttgg 361 tggttgacta cagacaaaaa gctgctgcag caggtagaat tcccacaaac tatctgagaa 421 gagaggttgg tacttctgat aaagaaattc taacaagtcg aataatagat cgttcaatta 481 gaccgctctt tccagctggc tacttctatg atacacaggt tctgtgtaat ctgttagcag 541 tagatggtgt aaatgagcct gatgtcctag caattaatgg cgcttccgta gccctctcat 601 tatcagatat tccttggaat ggacctgttg gggcagtacg aataggaata attgatggag 661 aatatgttgt taacccaaca agaaaagaaa tgtcttctag tactttaaat ttagtggttg 721 ctggagcacc taaaagtcag attgtcatgt tggaagcctc tgcagagaac attttacagc 781 aggacttttg ccatgctatc aaagtgggag tgaaatatac ccaacaaata attcagggca 841 ttcagcagtt ggtaaaagaa actggtgtta ccaagaggac acctcagaag ttatttaccc 901 cttcgccaga gattgtgaaa tatactcata aacttgctat ggagagactc tatgcagttt 961 ttacagatta cgagcatgac aaagtttcca gagatgaagc tgttaacaaa ataagattag 1021 atacggagga acaactaaaa gaaaaatttc cagaagccga tccatatgaa ataatagaat 1081 ccttcaatgt tgttgcaaag gaagttttta gaagtattgt tttgaatgaa tacaaaaggt 1141 gcgatggtcg ggatttgact tcacttagga atgtaagttg tgaggtagat atgtttaaaa 1201 cccttcatgg atcagcatta tttcaaagag gacaaacaca ggtgctttgt accgttacat 1261 ttgattcatt agaatctggt attaagtcag atcaagttat aacagctata aatgggataa 1321 aagataaaaa tttcatgctg cactacgagt ttcctcctta tgcaactaat gaaattggca 1381 aagtcactgg tttaaataga agagaacttg ggcatggtgc tcttgctgag aaagctttgt 1441 atcctgttat tcccagagat tttcctttca ccataagagt tacatctgaa gtcctagagt 1501 caaatgggtc atcttctatg gcatctgcat gtggcggaag tttagcatta atggattcag 1561 gggttccaat ttcatctgct gttgcaggcg tagcaatagg attggtcacc aaaaccgatc 1621 ctgagaaggg tgaaatagaa gattatcgtt tgctgacaga tattttggga attgaagatt 1681 acaatggtga catggacttc aaaatagctg gcactaataa aggaataact gcattacagg 1741 ctgatattaa attacctgga ataccaataa aaattgtgat ggaggctatt caacaagctt 1801 cagtggcaaa aaaggagata ttacagatca tgaacaaaac tatttcaaaa cctcgagcat 1861 ctagaaaaga aaatggacct gttgtagaaa ctgttcaggt tccattatca aaacgagcaa 1921 aatttgttgg accTGGTGGC TATAACTTAA AAAAACTTCA GGCTGAAACA GGTGTAACTA 1981 TTAGTCAGGT GGATGAAGAA ACGTTTTCTG TATTTGCACC AACACCCAGT GCTATGCATG 2041 AGGCAAGAGA CTTCATTACT GAAATCTGCA AGGATGATCA GGAGCAGCAA TTAGAATTTG 2101 GAGCAGTATA TACCGCCACA ATAACTGAAA TCAGAGATAC TGGTGTAATG GTAAAATTAT 2161 ATCCAAATAT GACTGCGGTA CTGCTTCATA ACACACAACT TGATCAACGA AAGATTAAAC 2221 ATCCTACTGC CCTAGGATTA GAAGTTGGCC AAGAAATTCA GGTGAAATAC TTTGGACGTG 2281 ACCCAGCCGA TGGAAGAATG AGGCTTTCTC GAAAAGTGCT TCAGTCGCCA GCTACAACCG 2341 TGGTCAGAAC TTTGAATGAC AGAAGTAGTA TTGTAATGGG AGAACCTATT TCACAGTCAT 2401 CATCTAATTC TCAGTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2461 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2521 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TGTAACACCC CAATTATTCC 2581 GGCAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt