Construct: ORF TRCN0000477874
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018283.1_s317c1
- Derived from:
- ccsbBroadEn_03639
- DNA Barcode:
- CTCAGATCGGACTGATGGTTACTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IQCC (55721)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477874
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55721 | IQCC | IQ motif containing C | NM_018134.3 | 100% | 100% | |
| 2 | human | 55721 | IQCC | IQ motif containing C | NM_001160042.2 | 85.3% | 85.3% | 41_280del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1467
- ORF length:
- 1398
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagccagag ctgctggttc ggaaggtgtc tgcattgcag gcctgcgtcc 121 ggggcttctt ggtccgacgc cagttccaga gcctgcgagc tgagtatgag gcgattgtac 181 gagaggtcga gggcgacctg ggcacgcttc agtggaccga gggccgcatt cccaggccgc 241 gattcctccc agagaaggca aaatcccatc agacctggaa agcaggagac agggtagcaa 301 atccagagca ggggctgtgg aaccacttcc catgtgaaga gtctgaggga gaggccacct 361 gggaggagat ggtgctgaag aagtcaggag agagctcagc aaatcaagga agcctctgca 421 gagatcacag ctcctggctt cagatgaagc agaacaggaa acccagccaa gagaagacca 481 gagacacgac aaggatggag aatccagaag ccacagatca aagactgccc cacagccaac 541 ctcagcttca agagcttcag taccaccgca gccacttggc catggaattg ctgtggctgc 601 aacaggccat caatagccgt aaggagtacc tacttcttaa acaaacactg agatccccag 661 aggcgggccc gatcagagag gaaccccgcg tgttcctaga acatggggaa caggcctgtg 721 agagggacca gtcacaacca agcgcaccac tggaggacca gtcctacaga gacaggacca 781 ctggagagct agaacaggag gatgactcct gtcacagggt caaatcaccc cacagatccc 841 caggaagttt ggccactaca caaaaaaaca ttgctggggc taagtgcaga gaaccatgct 901 acagcaagtc tggaccaccg tcgtctatac catcaaacag ccaggccttg ggggacaggc 961 tcaccaaagg gccagacgat ggaagacaga cctttggagg gacctgcctg ctgcagatga 1021 aaaTCCTGGA GGACCAGACC CCCAGAGGTT TAAAACCTAG GAACCATTGT CCCAGGAAGT 1081 CCAGGACACA GCTGTCTGCA CTCTATGAGG ACTCAAATAT TAAGGAGATG TCTCCCAGAA 1141 AACTAGACCA CAAAGAGCCT GACTGCCGAA CAGTCAGGAC ACAAGAGTTG GGCCTCTCAG 1201 AGGACCACAT CATCTGGGAT GGTACCTTGG GGGGGCCAGA GCATAGTGTC CTCGATCTCT 1261 GGAGGACTAA ACCACCCAAA GGCCAGGCCC CCACTGATAG AAGCTCCAGA GATGGAACCT 1321 CCAATGAGCC TAGTCATGAA GGACAGAAAA AGCAGAGGAC TATACCATGG AGATCAAAGT 1381 CACCTGAGAT TCTGTCTTCT ACAAAGGCAG GCTGTACAGG AGAGGAACAG TGGAGGGGCA 1441 GGCCATGGAA AACAGAACCA CCTGGCTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 1501 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1561 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACTCAGATC 1621 GGACTGATGG TTACTCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1681 aagatt