Construct: ORF TRCN0000477879
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010251.1_s317c1
- Derived from:
- ccsbBroadEn_05533
- DNA Barcode:
- TGACCAATGGCAGAGCTTCAACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NCR3LG1 (374383)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477879
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 374383 | NCR3LG1 | natural killer cell cytotox... | NM_001202439.3 | 100% | 100% | |
| 2 | human | 374383 | NCR3LG1 | natural killer cell cytotox... | XM_011520072.3 | 93.6% | 93.6% | 0_1ins87 |
| 3 | human | 374383 | NCR3LG1 | natural killer cell cytotox... | XM_011520073.3 | 93.6% | 93.6% | 0_1ins87 |
| 4 | human | 374383 | NCR3LG1 | natural killer cell cytotox... | XM_011520074.3 | 93.6% | 93.6% | 0_1ins87 |
| 5 | human | 374383 | NCR3LG1 | natural killer cell cytotox... | XM_011520075.3 | 93.6% | 93.6% | 0_1ins87 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1431
- ORF length:
- 1362
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacgtggagg gctgccgcct ccacgtgcgc ggcgctcctg attctgctgt 121 gggcgctgac gaccgaaggt gatctgaaag tagagatgat ggcagggggg actcagatca 181 cacccctgaa tgacaatgtc accatattct gcaatatctt ttattcccaa cccctcaaca 241 tcacgtctat gggtatcacc tggttttgga agagtctgac gtttgacaaa gaagtcaaag 301 tctttgaatt ttttggagat caccaagagg cattccgacc tggagccatt gtgtctccat 361 ggaggctgaa gagtggggac gcctcactgc ggctgcctgg aatccagctg gaggaagcag 421 gagagtaccg atgtgaggtg gtggtcaccc ctctgaaggc acagggaaca gtccagcttg 481 aagttgtggc ttccccagcc agcagattgt tgctggatca agtgggcatg aaagagaatg 541 aagacaaata tatgtgtgag tcaagtgggt tctacccaga ggctattaat ataacatggg 601 agaagcagac ccagaagttt ccccatccca tagagatttc tgaggatgtc atcactggtc 661 ccaccatcaa gaatatggat ggcacattta atgtcactag ctgcttgaag ctgaactcct 721 ctcaggaaga ccctgggact gtctaccagt gtgtggtacg gcatgcgtcc ttgcataccc 781 ccttgaggag caactttacc ctgactgctg ctcggcacag tctttctgaa actgagaaga 841 cagataattt ttccattcat tggtggccta tttcattcat tggtgttgga ctggttttat 901 taattgtttt gattccttgg aaaaagatat gtaacaaatc atcttcagcc tatactcctc 961 tcaagtgcat tctgaaacac tggaactcct ttgacactca gactctgaag aaagagcacc 1021 tcatattctt ttgcactcgg gcatggccgt cttaccagct gcaggatggg gaggcttggc 1081 ctcctgaggg aagtgttaat attaatacta ttcaacaact agatgttttc tgcagacagg 1141 agggcaaatg gtccgaggtt ccTTATGTGC AAGCCTTCTT TGCCTTGCGA GACAACCCAG 1201 ATCTTTGTCA GTGTTGTAGA ATTGACCCTG CTCTCCTAAC AGTTACATCA GGCAAGTCCA 1261 TAGATGATAA TTCCACAAAG TCTGAGAAAC AAACCCCTAG GGAACACTCG GATGCAGTTC 1321 CGGATGCCCC AATCCTTCCT GTCTCCCCTA TCTGGGAACC TCCTCCAGCC ACAACATCAA 1381 CAACTCCAGT TCTATCCTCC CAACCCCCAA CTTTACTGTT ACCCCTACAG TTGCCAACTT 1441 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1501 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1561 CTTGTGGAAA GGACGATGAC CAATGGCAGA GCTTCAACCA ACGCGTTAAG TCgacaatca 1621 acctctggat tacaaaattt gtgaaagatt