Construct: ORF TRCN0000477886
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003267.1_s317c1
- Derived from:
- ccsbBroadEn_03112
- DNA Barcode:
- ACTCAACCGCTCCGCTATAGCCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NENF (29937)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477886
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29937 | NENF | neudesin neurotrophic factor | NM_013349.5 | 100% | 100% | |
| 2 | human | 29937 | NENF | neudesin neurotrophic factor | NR_026598.2 | 49.6% | 1_24del;200_201ins61;480_855del | |
| 3 | mouse | 66208 | Nenf | neuron derived neurotrophic... | NM_025424.2 | 88.1% | 90.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 582
- ORF length:
- 516
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gggccccgcg ccgcggcggc ggctgcggcc gctggcagcg ctggccctgg 121 tcctggcgct ggccccgggg ctgcccacag cccgggccgg gcagacaccg cgccctgccg 181 agcgggggcc cccagtgcgg cttttcaccg aggaggagct ggcccgctat ggcggggagg 241 aggaagatca gcccatcTAC TTGGCAGTGA AGGGAGTGGT GTTTGATGTC ACCTCCGGAA 301 AGGAGTTTTA TGGACGAGGA GCCCCCTACA ATGCCTTGAC GGGGAAGGAC TCCACTAGAG 361 GGGTAGCCAA GATGTCCTTG GATCCTGCAG ACCTCACCCA TGACACTACG GGTCTCACGG 421 CCAAGGAACT GGAGGCCCTG GATGAGGTCT TCACCAAAGT GTACAAAGCC AAATACCCCA 481 TCGTCGGCTA CACTGCCCGG AGAATTCTCA ATGAGGATGG CAGCCCTAAC CTGGACTTCA 541 AGCCTGAAGA CCAGCCCCAT TTTGACATCA AGGATGAGTT CTGCCCAACT TTCTTGTACA 601 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 661 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 721 AGGACGAACT CAACCGCTCC GCTATAGCCC CACGCGTTAA GTCgacaatc aacctctgga 781 ttacaaaatt tgtgaaagat t