Construct: ORF TRCN0000477899
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016979.1_s317c1
- Derived from:
- ccsbBroadEn_04290
- DNA Barcode:
- TCTACCTTGATCAAGGCTTGGAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- JAM3 (83700)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477899
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83700 | JAM3 | junctional adhesion molecule 3 | NM_032801.5 | 100% | 100% | |
| 2 | human | 83700 | JAM3 | junctional adhesion molecule 3 | NM_001205329.1 | 83.5% | 83.5% | 255_256ins153 |
| 3 | mouse | 83964 | Jam3 | junction adhesion molecule 3 | NM_023277.4 | 85.7% | 86.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 999
- ORF length:
- 930
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgctgagg cggccaccgc gactccggct ctgcgctcgg ctgcctgact 121 tcttcctgct gctgcttttc aggggctgcc tgataggggc tgtaaatctc aaatccagca 181 atcgaacccc agtggtacag gaatttgaaa gtgtggaact gtcttgcatc attacggatt 241 cgcagacaag tgaccccagg atcgagtgga agaaaattca agatgaacaa accacatatg 301 tgttttttga caacaaaatt cagggagact tggcgggtcg tgcagaaata ctggggaaga 361 catccctgaa gatctggaat gtgacacgga gagactcagc cctttatcgc tgtgaggtcg 421 ttgctcgaaa tgaccgcaag gaaattgatg agattgtgat cgagttaact gtgcaagtga 481 agccagtgac ccctgtctgt agagtgccga aGGCTGTACC AGTAGGCAAG ATGGCAACAC 541 TGCACTGCCA GGAGAGTGAG GGCCACCCCC GGCCTCACTA CAGCTGGTAT CGCAATGATG 601 TACCACTGCC CACGGATTCC AGAGCCAATC CCAGATTTCG CAATTCTTCT TTCCACTTAA 661 ACTCTGAAAC AGGCACTTTG GTGTTCACTG CTGTTCACAA GGACGACTCT GGGCAGTACT 721 ACTGCATTGC TTCCAATGAC GCAGGCTCAG CCAGGTGTGA GGAGCAGGAG ATGGAAGTCT 781 ATGACCTGAA CATTGGCGGA ATTATTGGGG GGGTTCTGGT TGTCCTTGCT GTACTGGCCC 841 TGATCACGTT GGGCATCTGC TGTGCATACA GACGTGGCTA CTTCATCAAC AATAAACAGG 901 ATGGAGAAAG TTACAAGAAC CCAGGGAAAC CAGATGGAGT TAACTACATC CGCACTGACG 961 AGGAGGGCGA CTTCAGACAC AAGTCATCGT TTGTGATCTT GCCAACTTTC TTGTACAAAG 1021 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1081 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1141 ACGATCTACC TTGATCAAGG CTTGGAAAAC GCGTTAAGTC gacaatcaac ctctggatta 1201 caaaatttgt gaaagatt