Construct: ORF TRCN0000477901
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005635.1_s317c1
- Derived from:
- ccsbBroadEn_02762
- DNA Barcode:
- CGCAGTTCCAGGCCTCCTGCTCCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- COMMD3 (23412)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477901
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23412 | COMMD3 | COMM domain containing 3 | NM_012071.4 | 99.8% | 29.7% | 164_165insC |
| 2 | human | 100532731 | COMMD3-BMI1 | COMMD3-BMI1 readthrough | NM_001204062.1 | 28.8% | 12.3% | (many diffs) |
| 3 | mouse | 12238 | Commd3 | COMM domain containing 3 | NM_147778.3 | 91.8% | 25.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 303
- ORF length:
- 234
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagctctcg gagtctgtgc agaaaggctt ccagatgctg gcggatcccc 121 gctccttcga ctccaacgcc ttcacgcttc tcctccgggc ggcattccag agtcTGCTGG 181 ACGCCCAGGC GGACGAGGCC GTGTTAGATC ATCCAGACTT GAAACATATC GACCCCAGTG 241 GTTTTAAAAC ATTGTCATGC AGCAGCTGCA ACTTACATAC TAGAGGCAGG AAAGCACCGA 301 GCTGACAAGT CAACTCTAAG CACTTATCTA GAAGACTGTA AATTTGACAG AGAGCGAATA 361 GAACTGTTTT GCACGGAATA TCAGAATAAT AAGAATTCCC TAGAAATCCT ACTGGGAAGT 421 ATAGGCAGAT CTCTCCCTCA TATAACGGAT GTTTCTTGGC GCTTGGAATA TCAGATAAAG 481 ACCAATCAAC TTCATAGGAT GTACAGACCT GCATATTTGG TGACCTTAAG TGTACAGAAC 541 ACTGATTCCC CATCCTATCC AGAGATTAGT TTTAGTTGCA GCATGGAACA ATTACAGGAC 601 TTGGTGGGGA AACTTAAAGA TGCTTCGAAA AGCCTGGAAA GAGCAACTCA GTTGTTGCCA 661 ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC 721 GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT 781 ATATCTTGTG GAAAGGACGA CGCAGTTCCA GGCCTCCTGC TCCGACGCGT TAAGTCgaca 841 atcaacctct ggattacaaa atttgtgaaa gatt