Construct: ORF TRCN0000477906
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015853.1_s317c1
- Derived from:
- ccsbBroadEn_12775
- DNA Barcode:
- TTGCTACCTCCATTACGACAATTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FBXO30 (84085)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477906
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84085 | FBXO30 | F-box protein 30 | NM_001348092.2 | 90.6% | 90.6% | 1_210del |
| 2 | human | 84085 | FBXO30 | F-box protein 30 | NM_032145.5 | 90.6% | 90.6% | 1_210del |
| 3 | human | 84085 | FBXO30 | F-box protein 30 | XM_011536177.3 | 90.6% | 90.6% | 1_210del |
| 4 | human | 84085 | FBXO30 | F-box protein 30 | XM_017011353.1 | 90.6% | 90.6% | 1_210del |
| 5 | mouse | 71865 | Fbxo30 | F-box protein 30 | NM_001168297.1 | 79.7% | 79% | (many diffs) |
| 6 | mouse | 71865 | Fbxo30 | F-box protein 30 | NM_027968.3 | 79.7% | 79% | (many diffs) |
| 7 | mouse | 71865 | Fbxo30 | F-box protein 30 | XM_006512859.3 | 79.7% | 79% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2091
- ORF length:
- 2025
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccgaaataaa gttgctgaac atctagaaat gtgtcctgca agtgtggtgt 121 gctgtactat ggaatggaat cgatggccag ttagttatgc agaccggaaa tcatatgaaa 181 atctaagcag agatgtcgat gaagtggcac aattggatat ggccttggct cttcaagacc 241 aaaggatgct cttagaatcc ctcaaagtag ccaccatgat gtcaaaagca actgataaag 301 tatccaaacc tagagaacaa atctcagtta aatcaagtgt cccagaaata ccacatgcta 361 atggtttagt gtctgttgat gaagaatctt atggtgcact ttatcaagct actgtagaaa 421 caaccagaag tttggctgct gctttggata tcctgaatac tgctacaaga gacattggca 481 tgttaaatac aagtgtccca aatgacatgg atgaacagca aaatgcgaga gaaagcttag 541 aggatcaaaa cttgaaagac caagatcatc tttatgagga ggaaatagga gcagtaggtg 601 gaattgacta caatgacaca aatcagaatg cccagtctga acaaaatggt tcaagtgatt 661 tattatgtga cttgaataca agttcttatg acacttctgc tctttgtaat ggctttcctt 721 tggaaaatat atgtacccag gtcatagacc agaatcagaa tttacatggt gattcaaaac 781 aaagtaactt aacaaatgga gactgtgtgg catcatcaga tggcacttca aaaccttcca 841 gctcacttgc ggtggcagca caacttaggg aaataatacc atccagtgct ttgcctaatg 901 gcacagttca gcatatcctc atgccagatg atgaaggtga aggtgaattg tgttggaaaa 961 aagtagactt aggggacgtg aagaatgtgg atgtcttatc tttcagtcat gctccttcat 1021 tcaattttct ttctaattca tgttggtcta aaccaaagga agataaagca gtagatacat 1081 cagatttgga agttgcagaa gatcctatgg gcctccaagg aatagatctg atcacagcag 1141 cattgctttt ttgtctagga gattctccag gagggagggg tatatctgat agccgcatgg 1201 ctgatattta tcacattgac gttgggactc agactttttc acttccatct gcaatattag 1261 ctacaagtac aatggttggg gagatagctt cagcttcagc ttgtgatcat gccaatccac 1321 agctttcaaa tccaagtccg tttcagacac ttgggctgga tttagtattg gaatgtgtcg 1381 ctaggtacca acccaagcag cgttcaatgt ttacctttgt gtgtggacag ttatttagaa 1441 ggaaagaatt ttcttcccac tttaagaatg tgcatggtga cattcatgct ggactcaatg 1501 gctggatgga acagaggtgc cctttagctt actatggttg tacctattct cagcgtagat 1561 tttgtccatc aatacaagga gcaaagatta tacatgaccg ccatttgagg tcatttggag 1621 ttcagccatg tgtatctaca gtattagtgg agcctgctag aaactgtgtg ttgggattac 1681 ataatgacca tctaagtagt cttccttttg aggTCCTGCA GCATATTGCA GGCTTTCTCG 1741 ATGGCTTCAG CTTATGTCAG CTCTCATGTG TATCCAAGTT AATGAGGGAT GTGTGTGGCA 1801 GCCTGCTTCA GTCTCGTGGC ATGGTCATAC TGCAGTGGGG GAAAAGGAAG TATCCAGAAG 1861 GAAATTCATC ATGGCAGATA AAAGAAAAGG TATGGCGATT TAGTACTGCA TTTTGTTCTG 1921 TTAATGAATG GAAATTTGCT GACATCCTAA GCATGGCAGA CCACTTGAAG AAATGCAGTT 1981 ACAATGTTGT CGAGAAACGG GAGGAAGCAA TCCCTTTGCC ATGTATGTGT GTGACACGAG 2041 AACTCACTAA AGAAGGACGT TCACTACGCT CAGTTTTAAA ACCTGTACTT TACCCAACTT 2101 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2161 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2221 CTTGTGGAAA GGACGATTGC TACCTCCATT ACGACAATTG ACGCGTTAAG TCgacaatca 2281 acctctggat tacaaaattt gtgaaagatt