Construct: ORF TRCN0000477910

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003217.1_s317c1
Derived from:
ccsbBroadEn_04333
DNA Barcode:
TTTACTCAGAAGTACATTCGAGGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
USP42 (84132)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477910
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 84132 USP42 ubiquitin specific peptidas... NM_032172.3 100% 100%
2 human 84132 USP42 ubiquitin specific peptidas... NM_001365764.1 99.3% 99.3% 3946_3952delAAGAGGA;3956_3972del
3 human 84132 USP42 ubiquitin specific peptidas... XM_006715790.2 99% 99% 3942_3943insGGT;3946_3981del
4 human 84132 USP42 ubiquitin specific peptidas... XM_006715791.2 99% 99% 3942_3943insGGT;3946_3981del
5 human 84132 USP42 ubiquitin specific peptidas... XM_011515573.1 99% 99% 3942_3943insGGT;3946_3981del
6 human 84132 USP42 ubiquitin specific peptidas... XM_024446968.1 99% 99% 3942_3943insGGT;3946_3981del
7 human 84132 USP42 ubiquitin specific peptidas... XM_024446969.1 99% 99% 3942_3943insGGT;3946_3981del
8 human 84132 USP42 ubiquitin specific peptidas... XM_011515574.1 89.8% 87.2% (many diffs)
9 human 84132 USP42 ubiquitin specific peptidas... XM_011515577.1 87.9% 87.8% (many diffs)
10 human 84132 USP42 ubiquitin specific peptidas... XM_011515578.1 87.4% 87.4% 0_1ins462;3480_3481insGGT;3484_3519del
11 human 84132 USP42 ubiquitin specific peptidas... XM_005249883.5 55.8% 55.7% (many diffs)
12 human 84132 USP42 ubiquitin specific peptidas... XR_002956494.1 52.9% 1_67del;4013_4019delAAGAGGA;4023_7458del
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
4017
ORF length:
3948
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gaccatagtt gacaaagctt ctgaatcttc agacccatca gcctatcaga
121atcagcctgg cagctccgag gcagtctcac ctggagacat ggatgcaggt tctgccagct
181ggggtgctgt gtcttcattg aatgatgtgt caaatcacac actttcttta ggaccagtac
241ctggtgctgt agtttattcg agttcatctg tacctgataa atcaaaacca tcaccacaaa
301aggatcaagc cctaggtgat ggcatcgctc ctccacagaa agttcttttc ccatctgaga
361agatttgtct taagtggcaa caaactcata gagttggagc tgggctccag aatttgggca
421atacctgttt tgccaatgca gcactgcagt gtttaaccta cacaccacct cttgccaatt
481acatgctatc acatgaacac tccaaaacat gtcatgcaga aggcttttgt atgatgtgta
541caatgcaagc acatattacc caggcactca gtaatcctgg ggacgttatt aaaccaatgt
601ttgtcatcaa tgagatgcgg cgtatagcta ggcacttccg ttttggaaac caagaagatg
661cccatgaatt ccttcaatac actgttgatg ctatgcagaa agcatgcttg aatggcagca
721ataaattaga cagacacacc caggccacca ctcttgtttg tcagatattt ggaggatacc
781taagatctag agtcaaatgt ttaaattgca agggcgtttc agatactttt gatccatatc
841ttgatataac attggagata aaggctgctc agagtgtcaa caaggcattg gagcagtttg
901tgaagccgga acagcttgat ggagaaaact cgtacaagtg cagcaagtgt aaaaagatgg
961ttccagcttc aaagaggttc actatccata gatcctctaa tgttcttaca ctttctctga
1021aacgttttgc aaattttacc ggtggaaaaa ttgctaagga tgtgaaatac cctgagtatc
1081ttgatattcg gccatatatg tctcaaccca acggagagcc aattgtctac gtcttgtatg
1141cagtgctggt ccacactggt tttaattgcc atgctggcca ttacttctgc tacataaaag
1201ctagcaatgg cctctggtat caaatgaatg actccattgt atctaccagt gatattagat
1261cggtactcag ccaacaagcc tatgtgctct tttatatcag gtcccatgat gtgaaaaatg
1321gaggtgaact tactcatccc acccatagcc ccggccagtc ctctccccgc cccgtcatca
1381gtcagcgggt tgtcaccaac aaacaggctg cgccaggctt tatcggacca cagcttccct
1441ctcacatgat aaagaatcca cctcacttaa atgggactgg accattgaaa gacacgccaa
1501gcagttccat gtcgagtcct aacgggaatt ccagtgtcaa cagggctagt cctgttaatg
1561cttcagcttc tgtccaaaac tggtcagtta ataggtcctc agtgatccca gaacatccta
1621agaaacaaaa aattacaatc agtattcaca acaagttgcc tgttcgccag tgtcagtctc
1681aacctaacct tcatagtaat tctttggaga accctaccaa gcccgttccc tcttctacca
1741ttaccaattc tgcagtacag tctacctcga acgcatctac gatgtcagtt tctagtaaag
1801taacaaaacc gatcccccgc agtgaatcct gctcccagcc cgtgatgaat ggcaaatcca
1861agctgaactc cagcgtgctg gtgccctatg gcgccgagtc ctctgaggac tctgacgagg
1921agtcaaaggg gctgggcaag gagaatggga ttggtacgat tgtgagctcc cactctcccg
1981gccaagatgc cgaagatgag gaggccactc cgcacgagct tcaagaaccc atgaccctaa
2041acggtgctaa tagtgcagac agcgacagtg acccgaaaga aaacggccta gcgcctgatg
2101gtgccagctg ccaaggccag cctgccctgc actcagaaaa tccctttgct aaggcaaacg
2161gtcttcctgg aaagttgatg cctgctcctt tgctgtctct cccagaagac aaaatcttag
2221agaccttcag gcttagcaac aaactgaaag gctcgacgga tgaaatgagt gcacctggag
2281cagagagggg ccctcccgag gaccgcgacg ccgagcctca gcctggcagc cccgccgccg
2341aatccctgga ggagccagat gcggccgccg gcctcagcag caccaagaag gctccgccgc
2401cccgcgatcc cggcaccccc gctaccaaag aaggcgcctg ggaggccatg gccgtcgccc
2461ccgaggagcc tccgcccagc gccggcgagg acatcgtggg ggacacagca ccccctgacc
2521tgtgtgatcc cgggagctta acaggcgatg cgagcccgtt gtcccaggac gcaaagggga
2581tgatcgcgga gggcccgcgg gactcggcgt tggcggaagc cccggaaggg ttgagtccgg
2641ctccgcctgc gcggtcggag gagccctgcg agcagccact ccttgttcac cccagcgggg
2701accacgcccg ggacgctcag gacccatccc agagcttggg cgcacccgag gccgcagagc
2761ggccgccagc tcctgtgctg gacatggccc cggccggtca cccggaaggg gacgctgagc
2821ctagccccgg cgagagggtc gaggacgccg cggcgccgaa agccccaggc ccttccccag
2881cgaaggagaa aatcggcagc ctcagaaagg tggaccgagg ccactaccgc agccggagag
2941agcgctcgtc cagcggggag cccgccagag agagcaggag caagactgag ggccaccgtc
3001accggcggcg ccgcacctgc ccccgggagc gcgaccgcca ggaccgccac gccccggagc
3061accaccccgg ccacggcgac aggctcagcc ctggcgagcg ccgctctctg ggcaggtgca
3121gtcaccacca ctcccgacac cggagcgggg tggagctgga ctgggtcaga caccactaca
3181ccgagggcga gcgtggctgg ggccgggaga agttctaccc cgacaggccg cgctgggaca
3241ggtgccggta ctaccatgac aggtacgccc tgtacgctgc ccgggactgg aagcccttcc
3301acggcggccg cgagcacgag cgggccgggc tgcacgagcg gccgcacaag gaccacaacc
3361ggggccgtag gggctgcgag ccggcccggg agagggagcg gcaccgcccc agcagccccc
3421gcgcaggcgc gccccacgcc ctcgccccgc accccgaccg cttctcccac gacagaactg
3481cacttgtagc cggagacaac tgtaacctct ctgatcGGTT TCACGAACAC GAAAATGGAA
3541AGTCCCGGAA ACGGAGACAC GACAGTGTGG AGAACAGTGA CAGTCATGTT GAAAAGAAAG
3601CCCGGAGGAG CGAACAGAAG GATCCTCTAG AAGAGCCTAA AGCAAAGAAG CACAAAAAAT
3661CAAAGAAGAA AAAGAAATCC AAAGACAAAC ACCGAGACCG CGACTCCAGG CATCAGCAGG
3721ACTCAGACCT CTCAGCAGCG TGCTCTGACG CTGACCTCCA CAGACACAAA AAAAAGAAGA
3781AGAAAAAGAA GAGACATTCA AGAAAATCAG AGGACTTTGT TAAAGATTCA GAACTGCACT
3841TACCCAGGGT CACCAGCTTG GAGACTGTCG CCCAGTTCCG GAGAGCCCAG GGTGGCTTTC
3901CTCTCTCTGG TGGCCCGCCT CTGGAAGGCG TCGGACCTTT CCGTGAGAAA ACGAAACACT
3961TACGGATGGA AAGCAGGGAT GACAGGTGTC GTCTCTTTGA GTATGGCCAG GGTGATTTGC
4021CTACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
4081TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
4141ATATATCTTG TGGAAAGGAC GATTTACTCA GAAGTACATT CGAGGTACGC GTTAAGTCga
4201caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)