Construct: ORF TRCN0000477935
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001363.1_s317c1
- Derived from:
- ccsbBroadEn_10553
- DNA Barcode:
- CGAGGCAAAGTAATCGCCAAAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF542P (147947)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477935
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 147947 | ZNF542P | zinc finger protein 542, ps... | NR_024055.2 | 21.1% | 1_830del;1554_3412del | |
| 2 | human | 147947 | ZNF542P | zinc finger protein 542, ps... | NR_003127.2 | 20.6% | 1_927del;1651_3509del | |
| 3 | human | 147947 | ZNF542P | zinc finger protein 542, ps... | NR_024057.2 | 20% | 1_1011del;1735_3612del | |
| 4 | human | 147947 | ZNF542P | zinc finger protein 542, ps... | NR_024056.2 | 19.6% | 1_1083del;1807_3684del | |
| 5 | human | 147947 | ZNF542P | zinc finger protein 542, ps... | NR_033418.1 | 19.2% | 1_1151del;1875_3752del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 792
- ORF length:
- 723
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gactcatctc acacagcatc agactacaca tacgagagaa aagttccatg 121 aatgcagtga atgtggaaag gccttcagcc gtgtctcagc tcttatagat caccagcgaa 181 ttcatagtgg agaaaagccg tatgaatgta aggagtgtgg aagagccttc actcaaagtg 241 cccagctcat tagacatcag aaaactcatt ctggagaaaa accctatgag tgtagtaagt 301 gtaagaaatc ttttgtgcac ctgtcttccc tGATTGAACA TTGGAGAATT CACACTGGAG 361 AAAAACCATA TCAATGTAAG GACTGCAAAA AGACCTTTTG TCGTGTGATG CAGTTCACTC 421 TGCACAGGAG AATTCATACT GGTGAAAAAC CCTATGAATG CAAGGAATGT GGAAAGTCCT 481 TCAGCGCCCA TTCTTCTCTT GTTACTCATA AGAGAACACA CAGTGGAGAA AAACCGTATA 541 AATGCAAGGA ATGTGGAAAA GCCTTCAGTG CGCACTCTTC CCTTGTTACT CATAAGAGAA 601 CACACAGTGG AGAGAAACCC TATACATGCC ATGCCTGTGG GAAGGCCTTT AATACTTCCT 661 CCACACTTTG TCAACATAAT AGAATTCATA CTGGTGAAAA ACCCTTTCAG TGCAGTCAAT 721 GCGGGAAGTC CTTTAGCTGC AGCTCTCACC TTACTCGACA CTGTAGAATG TGTAATGGAA 781 AATTTAGCAA ATTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 841 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 901 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACGA GGCAAAGTAA TCGCCAAAAT 961 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t