Construct: ORF TRCN0000477948

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017667.1_s317c1
Derived from:
ccsbBroadEn_08631
DNA Barcode:
TTCGGGTTGAACGATACGTTCCTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PCDHA8 (56140)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477948
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 56140 PCDHA8 protocadherin alpha 8 NM_031856.1 99.8% 99.6% 233G>A;1251A>C;1735A>G
2 human 56142 PCDHA6 protocadherin alpha 6 NM_031848.3 89.7% 87.7% (many diffs)
3 human 56140 PCDHA8 protocadherin alpha 8 NM_018911.3 84.8% 83.1% (many diffs)
4 human 56142 PCDHA6 protocadherin alpha 6 NM_018909.4 77.4% 74.3% (many diffs)
5 human 56142 PCDHA6 protocadherin alpha 6 NM_031849.3 71.6% 57.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2511
ORF length:
2442
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggattatcac tggcgaggag agctgggatc ctggcgacta ctactcttgc
121ttctgctcct cgcagcctgg aaggtgggga gcggccagct ccactactcc gtccccgagg
181aggccaaaca cggcaccttc gtgggccgga tcgcgcagga cctggggctg gagctggcgg
241agctggtgcc gcgcctgttc cgggtggcgt ccaaaagaca ccgggacctt ctggaggtaa
301atctgcagaa tggcattttg tttgtgaatt ctcggatcga ccgcgaggag ctgtgcgggc
361ggagcgcgga gtgcagcatc cacctggagg tgatcgtgga caggccgctg caggttttcc
421atgtggacgt ggaggtgaag gatgttaatg acaacccgcc agtgttccgg gtaaaagacc
481aaaagctgtt tgtttcagaa tccagaatgc cagactctcg gtttccgcta gagggcgcgt
541ccgatgcaga tgttggagct aactccgtgt taacctacag gcttagctct catgattact
601tcatgctaga tgtgaattca aagaacgatg agaataaact ggttgagctc gtattaagaa
661aatccttgga cagagaggac gctcctgcgc accacttatt cctgacagcc acagatgggg
721gcaaacctga gctcacaggc actgttcagc tgctggtcac agtgctggat gtgaatgata
781atgctcccac tttcgaacag tctgaatacg aagtaagaat attcgaaaac gcagacaacg
841gaacaacagt tatcaaactg aatgcttctg atccggatga aggagccaat ggggcaattt
901catattcttt taatagcctt gttgaaacta tggttattga ccactttagc atagatcgaa
961atacgggaga aatagtgatt cggggtaatt tggattttga acaagaaaac ttatacaaaa
1021tcctcattga cgccacggac aaaggccatc ctcccatggc gggtcattgc accgttttag
1081tgagaatttt ggataaaaat gataacgtcc ctgagatagc actgacttcc ttatccttgc
1141ctgtacgtga agacgctcaa tttggtactg tcatcgccct aattagcgtg aacgacctcg
1201attcaggtgc caacgggcag gtgacctgct ccctgatgcc ccatgtcccc ttcaagctgg
1261tgtccacctt caagaattac tactcgttgg tgctggacag cgccctggac cgcgagagcg
1321tgtcggccta tgagttggtg gtaaccgcgc gggacggggg ctcgccttcg ctgtgggcca
1381ccgccagctt gtctgtggag gtggccgacg tgaacgacaa tgctccggcg ttcgcgcagc
1441ccgagtacac ggtgttcgtg aaggagaaca acccgccggg ctgccacatc ttcacggtgt
1501ctgcgcgaga cgcggacgcg caggagaacg cgctggtgtc ctactcgctt gtggagcggc
1561gggtgggcga gcgctcgctg tcgagctaca tttcggtgca cacggagagc ggcaaggtgt
1621acgcgctgca gccgctggac cacgaggagc tagagctgct gcagttccag gtgagcgcgc
1681gcgacgcggg cgtgccgcct ctgggcagca acgtgacgct gcaggtgttc gtgctggacg
1741agaatgacaa cgcgccggca ctgctggagc ctcgggtggg tggcactggt ggcgcagcga
1801gcgagctggt gccgcggtct gtgggcgcgg gccacgtggt agcgaaggtg cgcgcagtgg
1861acgccgactc gggctacaac gcgtggcttt cgtatgagct gcagccagct gcaagcagcc
1921ctcgcatccc gttccgcgtg gggctgtaca cgggcgagat cagcaccact cgtgtcctgg
1981acgaagcgga ctctccgcgc caccgtctgc tggtcctggt gaaggatcat ggtgaacctg
2041cgctgaccgc cacggccacg gttctggtgt cgctggtgga gagcggccag gctccaaaag
2101cgtcatcgag gcagtcggct ggcgttttgg gtccggaagc ggcgctggtg gatgtcaacg
2161tgtacctgat catcgccatc tgcgcggtat ccagcctgct ggtgctcacg ctgctgctgt
2221acactgcgcT GCGGTGCTCA GCACTGCCCA CTGAGGGCGG GTGCCGGGCG GGCAAGCCCA
2281CTCTGGTGTG CTCCAGTGCG GTGGGGAGCT GGTCATACTC GCAACAACAG CCGCAGAGGG
2341TGTGCTCTGG TGAGGGGCCA CCGAAGACGG ACCTCATGGC CTTCAGCCCC TGCCTTCCTC
2401CTGATCTGGG ATCAGTTGAT GTAGGCGAAG AGCAAGATTT AAATGTTGAT CATGGCCTCA
2461AAGTAAGTCC ATTTAAATTT AGAACTCATA AATTCTATTT GTGGAAATTG TTGCCAACTT
2521TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
2581CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
2641CTTGTGGAAA GGACGATTCG GGTTGAACGA TACGTTCCTT ACGCGTTAAG TCgacaatca
2701acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)