Construct: ORF TRCN0000477960
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017940.1_s317c1
- Derived from:
- ccsbBroadEn_13368
- DNA Barcode:
- GGGTGCTAACACTTGGCTTAGTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AMN1 (196394)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477960
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 196394 | AMN1 | antagonist of mitotic exit ... | XM_006719054.3 | 96.2% | 94.4% | (many diffs) |
| 2 | human | 196394 | AMN1 | antagonist of mitotic exit ... | NM_001278411.1 | 89.5% | 89.5% | 1_75del |
| 3 | human | 196394 | AMN1 | antagonist of mitotic exit ... | NM_001278412.1 | 89.5% | 89.5% | 1_75del |
| 4 | human | 196394 | AMN1 | antagonist of mitotic exit ... | XM_017018963.2 | 89.5% | 89.5% | 1_75del |
| 5 | human | 196394 | AMN1 | antagonist of mitotic exit ... | XM_017018964.1 | 89.5% | 89.5% | 1_75del |
| 6 | human | 196394 | AMN1 | antagonist of mitotic exit ... | XM_017018965.2 | 89.5% | 89.5% | 1_75del |
| 7 | human | 196394 | AMN1 | antagonist of mitotic exit ... | NM_001113402.2 | 83.3% | 83.3% | 1_129del |
| 8 | human | 196394 | AMN1 | antagonist of mitotic exit ... | NR_103523.1 | 31.7% | (many diffs) | |
| 9 | human | 196394 | AMN1 | antagonist of mitotic exit ... | NR_103522.1 | 27.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 714
- ORF length:
- 645
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagtatgcag ggacagataa cagattcaaa tataagtgag attttacatc 121 ctgaagtcca aactctagat ctacggagct gcgatatatc agatgctgct ctcctgcacc 181 tgtctaactg tagaaaactg aagaaattaa atttaaatgc ttcaaaaggg aaccgagttt 241 ctgtaacttc agaaggaata aaagctgtgg cttcatcttg ttcataccta cacgaagctt 301 ctttgaaaag atgctgcaat ctcactgacg aaggagtcgt tgcTCTTGCA CTCAATTGCC 361 AGCTGCTAAA GATCATCGAT TTAGGTGGCT GCTTAAGTAT TACTGATGTG TCCTTACATG 421 CATTAGGAAA AAACTGCCCA TTTTTGCAGT GTGTCGACTT TTCAGCTACT CAGGTATCTG 481 ACAGTGGTGT GATTGCACTT GTTAGTGGAC CTTGTGCGAA GAAATTAGAG GAGATTCATA 541 TGGGACATTG TGTAAATCTG ACTGATGGGG CTGTCGAAGC TGTCCTTACT TACTGTCCTC 601 AAATACGTAT ATTACTCTTC CATGGATGCC CCTTGATAAC AGATCATTCC CGAGAAGTGT 661 TGGAGCAATT AGTAGGCCCA AACAAACTAA AGCAAGTGAC ATGGACTGTT TATTTGCCAA 721 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 781 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 841 TATCTTGTGG AAAGGACGAG GGTGCTAACA CTTGGCTTAG TACACGCGTT AAGTCgacaa 901 tcaacctctg gattacaaaa tttgtgaaag att