Construct: ORF TRCN0000477982

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006492.1_s317c1
Derived from:
ccsbBroadEn_00269
DNA Barcode:
CGGTAATATTTAACCAAGACTTTA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ADGRE5 (976)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477982
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 976 ADGRE5 adhesion G protein-coupled ... NM_001784.5 100% 100%
2 human 976 ADGRE5 adhesion G protein-coupled ... NM_001025160.2 94.4% 94.4% 345_476del
3 human 976 ADGRE5 adhesion G protein-coupled ... NM_078481.4 88.8% 88.8% 344_622del
4 human 976 ADGRE5 adhesion G protein-coupled ... XM_011528451.3 87.8% 87.7% 344_622del;2479_2480delAAinsGC;2481_2482ins24
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2292
ORF length:
2226
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg aggccgcgtc tttctcgcat tctgtgtctg gctgactctg ccgggagctg
121aaacccagga ctccaggggc tgtgcccggt ggtgccctca gaactcctcg tgtgtcaatg
181ccaccgcctg tcgctgcaat ccagggttca gctctttttc tgagatcatc accaccccga
241cggagacttg tgacgacatc aacgagtgtg caacaccgtc gaaagtgtca tgcggaaaat
301tctcggactg ctggaacaca gaggggagct acgactgcgt gtgcagcccg ggatatgagc
361ctgtttctgg ggcaaaaaca ttcaagaatg agagcgagaa cacctgtcaa gatgtggacg
421agtgcagctc cgggcagcat cagtgtgaca gctccaccgt ctgcttcaac accgtgggtt
481catacagctg ccgctgccgc ccaggctgga agcccagaca cggaatcccg aataaccaaa
541aggacactgt ctgtgaagat atgactttct ccacctggac cccgccccct ggagtccaca
601gccagacgct ttcccgattc ttcgacaaag tccaggacct gggcagagac tccaagacaa
661gctcagccga ggtcaccatc cagaatgtca tcaaattggt ggatgaactg atggaagctc
721ctggagacgt agaggccctg gcgccacctg tccggcacct catagccacc cagctgctct
781caaaccttga agatatcatg aggatcctgg ccaagagcct gcctaaaggc cccttcacct
841acatttcccc ttcgaacaca gagctgaccc tgatgatcca ggagcggggg gacaagaacg
901tcactatggg tcagagcagc gcacgcatga agctgaattg ggctgtggca gctggagccg
961aggatccagg ccccgccgtg gcgggcatcc tctccatcca gaacatgacg acattgctgg
1021ccaatgcctc cttgaacctg cattccaaga agcaagccga actggaggag atatatgaaa
1081gcagcatccg tggtgtccaa ctcagacgcc tctctgccgt caactccatc tttctgagcc
1141acaacaacac caaggaactc aactccccca tccttttcgc cttctcccac cttgagtcct
1201ccgatgggga ggcgggaaga gaccctcctg ccaaggacgt gatgcctggg ccacggcagg
1261agctgctctg tgccttctgg aagagtgaca gcgacagggg agggcactgg gccaccgagg
1321gctgccaggt gctgggcagc aagaacggca gcaccacctg ccaatgcagc cacctgagca
1381gctttgcgat ccttatggct cattatgacg tggaggactg gaagctgacc ctgatcacca
1441gggtgggact ggcgctgtca ctcttctgcc tgctgctgtg catcctcact ttcctgctgg
1501tgcggcccat ccagggctcg cgcaccacca tacacctgca cctctgcatc tgcctcttcg
1561tgggctccac catcttcctg gccggcatcg agaacgaagg cggccaggtg gggctgcgct
1621gccgcctggt ggccgggctg ctgcactact gtttcctggc cgccttctgc tggatgagcc
1681tcgaaggcct ggagctctac tttcttgtgg tgcgcgtgtt ccaaggccag ggcctgagta
1741cgcgctggct ctgcctgatc ggctatggcg tgcccctgct catcgtgggc gtctcggctg
1801ccatctacag caagggctac ggccgcccca gatactgctg gttggacttt gagcagggct
1861tcctctggag cttcttggga cctgtgacct tcatcatttt gtgcaatgct gtcattTTCG
1921TGACTACCGT CTGGAAGCTC ACTCAGAAGT TTTCTGAAAT CAATCCAGAC ATGAAGAAAT
1981TAAAGAAGGC GAGGGCGCTG ACCATCACGG CCATCGCGCA GCTCTTCCTG TTGGGCTGCA
2041CCTGGGTCTT TGGCCTGTTC ATCTTCGACG ATCGGAGCTT GGTGCTGACC TATGTGTTTA
2101CCATCCTCAA CTGCCTGCAG GGCGCCTTCC TCTACCTGCT GCACTGCCTG CTCAACAAGA
2161AGGTTCGGGA AGAATACCGG AAGTGGGCCT GCCTAGTTGC TGGGGGGAGC AAGTACTCAG
2221AATTCACCTC CACCACGTCT GGCACTGGCC ACAATCAGAC CCGGGCCCTC AGGGCATCAG
2281AGTCCGGCAT ATGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
2341ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
2401CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACGG TAATATTTAA CCAAGACTTT
2461AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)