Construct: ORF TRCN0000477982
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006492.1_s317c1
- Derived from:
- ccsbBroadEn_00269
- DNA Barcode:
- CGGTAATATTTAACCAAGACTTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADGRE5 (976)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477982
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 976 | ADGRE5 | adhesion G protein-coupled ... | NM_001784.5 | 100% | 100% | |
| 2 | human | 976 | ADGRE5 | adhesion G protein-coupled ... | NM_001025160.2 | 94.4% | 94.4% | 345_476del |
| 3 | human | 976 | ADGRE5 | adhesion G protein-coupled ... | NM_078481.4 | 88.8% | 88.8% | 344_622del |
| 4 | human | 976 | ADGRE5 | adhesion G protein-coupled ... | XM_011528451.3 | 87.8% | 87.7% | 344_622del;2479_2480delAAinsGC;2481_2482ins24 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2292
- ORF length:
- 2226
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg aggccgcgtc tttctcgcat tctgtgtctg gctgactctg ccgggagctg 121 aaacccagga ctccaggggc tgtgcccggt ggtgccctca gaactcctcg tgtgtcaatg 181 ccaccgcctg tcgctgcaat ccagggttca gctctttttc tgagatcatc accaccccga 241 cggagacttg tgacgacatc aacgagtgtg caacaccgtc gaaagtgtca tgcggaaaat 301 tctcggactg ctggaacaca gaggggagct acgactgcgt gtgcagcccg ggatatgagc 361 ctgtttctgg ggcaaaaaca ttcaagaatg agagcgagaa cacctgtcaa gatgtggacg 421 agtgcagctc cgggcagcat cagtgtgaca gctccaccgt ctgcttcaac accgtgggtt 481 catacagctg ccgctgccgc ccaggctgga agcccagaca cggaatcccg aataaccaaa 541 aggacactgt ctgtgaagat atgactttct ccacctggac cccgccccct ggagtccaca 601 gccagacgct ttcccgattc ttcgacaaag tccaggacct gggcagagac tccaagacaa 661 gctcagccga ggtcaccatc cagaatgtca tcaaattggt ggatgaactg atggaagctc 721 ctggagacgt agaggccctg gcgccacctg tccggcacct catagccacc cagctgctct 781 caaaccttga agatatcatg aggatcctgg ccaagagcct gcctaaaggc cccttcacct 841 acatttcccc ttcgaacaca gagctgaccc tgatgatcca ggagcggggg gacaagaacg 901 tcactatggg tcagagcagc gcacgcatga agctgaattg ggctgtggca gctggagccg 961 aggatccagg ccccgccgtg gcgggcatcc tctccatcca gaacatgacg acattgctgg 1021 ccaatgcctc cttgaacctg cattccaaga agcaagccga actggaggag atatatgaaa 1081 gcagcatccg tggtgtccaa ctcagacgcc tctctgccgt caactccatc tttctgagcc 1141 acaacaacac caaggaactc aactccccca tccttttcgc cttctcccac cttgagtcct 1201 ccgatgggga ggcgggaaga gaccctcctg ccaaggacgt gatgcctggg ccacggcagg 1261 agctgctctg tgccttctgg aagagtgaca gcgacagggg agggcactgg gccaccgagg 1321 gctgccaggt gctgggcagc aagaacggca gcaccacctg ccaatgcagc cacctgagca 1381 gctttgcgat ccttatggct cattatgacg tggaggactg gaagctgacc ctgatcacca 1441 gggtgggact ggcgctgtca ctcttctgcc tgctgctgtg catcctcact ttcctgctgg 1501 tgcggcccat ccagggctcg cgcaccacca tacacctgca cctctgcatc tgcctcttcg 1561 tgggctccac catcttcctg gccggcatcg agaacgaagg cggccaggtg gggctgcgct 1621 gccgcctggt ggccgggctg ctgcactact gtttcctggc cgccttctgc tggatgagcc 1681 tcgaaggcct ggagctctac tttcttgtgg tgcgcgtgtt ccaaggccag ggcctgagta 1741 cgcgctggct ctgcctgatc ggctatggcg tgcccctgct catcgtgggc gtctcggctg 1801 ccatctacag caagggctac ggccgcccca gatactgctg gttggacttt gagcagggct 1861 tcctctggag cttcttggga cctgtgacct tcatcatttt gtgcaatgct gtcattTTCG 1921 TGACTACCGT CTGGAAGCTC ACTCAGAAGT TTTCTGAAAT CAATCCAGAC ATGAAGAAAT 1981 TAAAGAAGGC GAGGGCGCTG ACCATCACGG CCATCGCGCA GCTCTTCCTG TTGGGCTGCA 2041 CCTGGGTCTT TGGCCTGTTC ATCTTCGACG ATCGGAGCTT GGTGCTGACC TATGTGTTTA 2101 CCATCCTCAA CTGCCTGCAG GGCGCCTTCC TCTACCTGCT GCACTGCCTG CTCAACAAGA 2161 AGGTTCGGGA AGAATACCGG AAGTGGGCCT GCCTAGTTGC TGGGGGGAGC AAGTACTCAG 2221 AATTCACCTC CACCACGTCT GGCACTGGCC ACAATCAGAC CCGGGCCCTC AGGGCATCAG 2281 AGTCCGGCAT ATGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 2341 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 2401 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACGG TAATATTTAA CCAAGACTTT 2461 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t