Construct: ORF TRCN0000478022
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008909.1_s317c1
- Derived from:
- ccsbBroadEn_00493
- DNA Barcode:
- TACAATCTACTATTCCCAGCGAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CELA1 (1990)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478022
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1990 | CELA1 | chymotrypsin like elastase 1 | NM_001971.6 | 100% | 100% | |
| 2 | mouse | 109901 | Cela1 | chymotrypsin-like elastase ... | NM_033612.2 | 83.1% | 81.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 840
- ORF length:
- 774
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct ggtcctttat ggacacagca cccaggacct tccggaaacc aatgcccgcg 121 tagtcggagg gactgaggcc gggaggaatt cctggccctc tcagatttcc ctccagtacc 181 ggtctggagg ttcccggtat cacacctgtg gagggaccct tatcagacag aactgggtga 241 tgacagctgc tcactgcgtg gattaccaga agactttccg cgtggtggct ggagaccata 301 acctgagcca gaatgatggc actgagcagt acgtgagtgt gcagaagatc gtggtgcatc 361 catactggaa cagcgataac gtggctgccg gctatgacat cgccctgctg cgcctggccc 421 agagcgttac cctcaatagc tatgtccagc tgggtgttct gccccaggag ggagccatcc 481 tggctaacaa cagtccctgc tacatcacag gctggggcaa gaccaagacc aatgggcagc 541 tggcccagac ccTGCAGCAG GCTTACCTGC CCTCTGTGGA CTACGCCATC TGCTCCAGCT 601 CCTCCTACTG GGGCTCCACT GTGAAGAACA CCATGGTGTG TGCTGGTGGA GATGGAGTTC 661 GCTCTGGATG CCAGGGTGAC TCTGGGGGCC CCCTCCATTG CTTGGTGAAT GGCAAGTATT 721 CTGTCCATGG AGTGACCAGC TTTGTGTCCA GCCGGGGCTG TAATGTCTCC AGGAAGCCTA 781 CAGTCTTCAC CCAGGTCTCT GCTTACATCT CCTGGATAAA TAATGTCATC GCCTCCAACT 841 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 901 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 961 TTTATATATC TTGTGGAAAG GACGATACAA TCTACTATTC CCAGCGAGCA CGCGTTAAGT 1021 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt