Transcript: Mouse NM_033612.2

Mus musculus chymotrypsin-like elastase family, member 1 (Cela1), mRNA.

Source:
NCBI, updated 2017-05-21
Taxon:
Mus musculus (mouse)
Gene:
Cela1 (109901)
Length:
1124
CDS:
28..828

Additional Resources:

NCBI RefSeq record:
NM_033612.2
NBCI Gene record:
Cela1 (109901)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_033612.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000306394 GCATGGTGAACGGTCAGTATG pLKO_005 686 CDS 100% 10.800 15.120 N Cela1 n/a
2 TRCN0000092404 CCCATGACTTATCGAGTGGTT pLKO.1 253 CDS 100% 2.640 3.696 N Cela1 n/a
3 TRCN0000332347 CCCATGACTTATCGAGTGGTT pLKO_005 253 CDS 100% 2.640 3.696 N Cela1 n/a
4 TRCN0000092405 GCCAAGAGTGTTACACTCAAT pLKO.1 403 CDS 100% 4.950 3.960 N Cela1 n/a
5 TRCN0000331388 GGCCGAGCACTTGTCTAATAT pLKO_005 929 3UTR 100% 15.000 10.500 N Cela1 n/a
6 TRCN0000092406 CCAGAGTCTCTGCTTACATTT pLKO.1 776 CDS 100% 13.200 9.240 N Cela1 n/a
7 TRCN0000332348 CCAGAGTCTCTGCTTACATTT pLKO_005 776 CDS 100% 13.200 9.240 N Cela1 n/a
8 TRCN0000306393 TTTCCCTCCAGTACCAGTATG pLKO_005 152 CDS 100% 10.800 7.560 N Cela1 n/a
9 TRCN0000092403 GAAGTAAGCAAGAAGGAGTTA pLKO.1 887 3UTR 100% 4.950 3.465 N Cela1 n/a
10 TRCN0000092407 TGGAACAAGAACAACGTGGTT pLKO.1 352 CDS 100% 2.640 1.848 N Cela1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_033612.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00493 pDONR223 100% 83.1% 81.9% None (many diffs) n/a
2 ccsbBroad304_00493 pLX_304 0% 83.1% 81.9% V5 (many diffs) n/a
3 TRCN0000478022 TACAATCTACTATTCCCAGCGAGC pLX_317 37.5% 83.1% 81.9% V5 (many diffs) n/a
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