Construct: ORF TRCN0000478045
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010556.1_s317c1
- Derived from:
- ccsbBroadEn_01919
- DNA Barcode:
- ATCGCGTCCCTCCGAATACGGGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLA2G10 (8399)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478045
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8399 | PLA2G10 | phospholipase A2 group X | NM_003561.2 | 100% | 100% | |
| 2 | human | 100652777 | LOC100652777 | group 10 secretory phosphol... | XM_011522789.2 | 78.5% | 74.4% | (many diffs) |
| 3 | human | 8399 | PLA2G10 | phospholipase A2 group X | XM_011522696.3 | 77.3% | 72.7% | (many diffs) |
| 4 | human | 100652777 | LOC100652777 | group 10 secretory phosphol... | XM_011522790.3 | 75.8% | 72.8% | (many diffs) |
| 5 | human | 8399 | PLA2G10 | phospholipase A2 group X | XM_011522695.3 | 75.1% | 71.7% | (many diffs) |
| 6 | human | 8399 | PLA2G10 | phospholipase A2 group X | XM_011522697.2 | 74.3% | 72.1% | (many diffs) |
| 7 | human | 8399 | PLA2G10 | phospholipase A2 group X | XM_011522698.2 | 72.5% | 72.1% | 357T>A;358_359insCGGCAGAGAACAA;360_361ins122 |
| 8 | human | 101929894 | LOC101929894 | uncharacterized LOC101929894 | XR_001752369.1 | 51.2% | (many diffs) | |
| 9 | human | 8399 | PLA2G10 | phospholipase A2 group X | NR_133651.1 | 47.5% | 1_344del;699_814del;956_1040del | |
| 10 | human | 105371103 | LOC105371103 | uncharacterized LOC105371103 | XR_933124.3 | 44.2% | (many diffs) | |
| 11 | human | 105371098 | LOC105371098 | uncharacterized LOC105371098 | XR_933136.2 | 43.6% | (many diffs) | |
| 12 | human | 105376753 | LOC105376753 | uncharacterized LOC105376753 | XR_933151.2 | 43.6% | (many diffs) | |
| 13 | human | 105371098 | LOC105371098 | uncharacterized LOC105371098 | XR_951915.2 | 43.6% | (many diffs) | |
| 14 | human | 105376749 | LOC105376749 | uncharacterized LOC105376749 | XR_933120.2 | 43.4% | (many diffs) | |
| 15 | human | 100652777 | LOC100652777 | group 10 secretory phosphol... | XR_951914.2 | 43.4% | (many diffs) | |
| 16 | human | 105376749 | LOC105376749 | uncharacterized LOC105376749 | XR_933118.2 | 43.3% | (many diffs) | |
| 17 | human | 105371103 | LOC105371103 | uncharacterized LOC105371103 | XR_933122.2 | 43.3% | (many diffs) | |
| 18 | human | 100652777 | LOC100652777 | group 10 secretory phosphol... | XR_951912.2 | 43.3% | (many diffs) | |
| 19 | human | 105371103 | LOC105371103 | uncharacterized LOC105371103 | XR_951926.2 | 43.3% | (many diffs) | |
| 20 | human | 101928556 | LOC101928556 | uncharacterized LOC101928556 | XR_001752360.1 | 43.1% | (many diffs) | |
| 21 | human | 101928556 | LOC101928556 | uncharacterized LOC101928556 | XR_001752358.1 | 41.4% | (many diffs) | |
| 22 | human | 105376749 | LOC105376749 | uncharacterized LOC105376749 | XR_933119.1 | 37.9% | (many diffs) | |
| 23 | human | 100652777 | LOC100652777 | group 10 secretory phosphol... | XR_951913.1 | 37.9% | (many diffs) | |
| 24 | human | 100652777 | LOC100652777 | group 10 secretory phosphol... | XR_001756355.1 | 37.4% | (many diffs) | |
| 25 | human | 105371103 | LOC105371103 | uncharacterized LOC105371103 | XR_933123.1 | 35.6% | (many diffs) | |
| 26 | human | 105371103 | LOC105371103 | uncharacterized LOC105371103 | XR_951927.2 | 35.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 564
- ORF length:
- 495
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggggccgcta cctgtgtgcc tgccaatcat gctgctcctg ctactgccgt 121 cgctgctgct gctgctgctt ctacctggcc ccgggtccgg cgaggcctcc aggatattac 181 gtgtgcaccg gcgtgggatc ctggaactgg caggaactgt gggttgtgtt ggtccccgaa 241 cccccatcgc ctatatgaaa tatggttgct tttgtggctt gggaggccat ggccagcccc 301 gcgatgccat tgactggtgc tgccatggcc acgactgttg ttacactcga gctgaggagg 361 ccggctgcag ccccaagaca gagcgctact cctggcagtg cgtcaatcag agcgtcctgt 421 GCGGACCGGC AGAGAACAAA TGCCAAGAAC TGTTGTGCAA GTGTGACCAG GAGATTGCTA 481 ACTGCTTAGC CCAAACTGAG TACAACTTAA AGTACCTCTT CTACCCCCAG TTCCTATGTG 541 AGCCGGACTC GCCCAAGTGT GACTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 601 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 661 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA TCGCGTCCCT 721 CCGAATACGG GGAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 781 att