Construct: ORF TRCN0000478046
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008580.1_s317c1
- Derived from:
- ccsbBroadEn_08045
- DNA Barcode:
- TACACATTGTTGAACGCTATTTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NOX1 (27035)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478046
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27035 | NOX1 | NADPH oxidase 1 | NM_007052.5 | 99.9% | 100% | 765C>T |
| 2 | human | 27035 | NOX1 | NADPH oxidase 1 | NM_001271815.1 | 93.3% | 93.4% | 141_142ins111;654C>T |
| 3 | human | 27035 | NOX1 | NADPH oxidase 1 | NM_013955.3 | 91.2% | 91.3% | 765C>T;1296_1297ins147 |
| 4 | human | 27035 | NOX1 | NADPH oxidase 1 | XM_017029407.2 | 81.1% | 81.2% | 0_1ins318;447C>T |
| 5 | mouse | 237038 | Nox1 | NADPH oxidase 1 | NM_172203.2 | 86.1% | 84% | (many diffs) |
| 6 | mouse | 237038 | Nox1 | NADPH oxidase 1 | XM_006528515.3 | 82% | 80% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1761
- ORF length:
- 1692
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggaaactgg gtggttaacc actggttttc agttttgttt ctggttgttt 121 ggttagggct gaatgttttc ctgtttgtgg atgccttcct gaaatatgag aaggccgaca 181 aatactacta cacaagaaaa atccttgggt caacattggc ctgtgcccga gcgtctgctc 241 tctgcttgaa ttttaacagc acgctgatcc tgcttcctgt gtgtcgcaat ctgctgtcct 301 tcctgagggg cacctgctca ttttgcagcc gcacactgag aaagcaattg gatcacaacc 361 tcaccttcca caagctggtg gcctatatga tctgcctaca tacagctatt cacatcattg 421 cacacctgtt taactttgac tgctatagca gaagccgaca ggccacagat ggctcccttg 481 cctccattct ctccagccta tctcatgatg agaaaaaggg gggttcttgg ctaaatccca 541 tccagtcccg aaacacgaca gtggagtatg tgacattcac cagcattgct ggtctcactg 601 gagtgatcat gacaatagcc ttgattctca tggtaacttc agctactgag ttcatccgga 661 ggagttattt tgaagtcttc tggtatactc accacctttt tatcttctat atccttggct 721 tagggattca cggcattggt ggaattgtcc ggggtcaaac agaggagagc atgaatgaga 781 gtcatcctcg caagtgtgca gagtcttttg agatgtggga tgatcgtgac tctcactgta 841 ggcgccctaa gtttgaaggg catccccctg agtcttggaa gtggatcctt gcaccggtca 901 ttctttatat ctgtgaaagg atcctccggt tttaccgctc ccagcagaag gttgtgatta 961 ccaaggttgt tatgcaccca tccaaagttt tggaattgca gatgaacaag cgtggcttca 1021 gcatggaagt ggggcagtat atctttgtta attgcccctc aatctctctc ctggaatggc 1081 atccttttac tttgacctct gctccagagg aagatttctt ctccattcat atccgagcag 1141 caggggactg gacagaaaat ctcataaggg ctttcgaaca acaatattca ccaattccca 1201 ggattgaagt ggatggtccc tttggcacag ccagtgagga tgttttccag tatgaagtgg 1261 ctgtgctggt tGGAGCAGGA ATTGGGGTCA CCCCCTTTGC TTCTATCTTG AAATCCATCT 1321 GGTACAAATT CCAGTGTGCA GACCACAACC TCAAAACAAA AAAGATCTAT TTCTACTGGA 1381 TCTGCAGGGA GACAGGTGCC TTTTCCTGGT TCAACAACCT GTTGACTTCC CTGGAACAGG 1441 AGATGGAGGA ATTAGGCAAA GTGGGTTTTC TAAACTACCG TCTCTTCCTC ACCGGATGGG 1501 ACAGCAATAT TGTTGGTCAT GCAGCATTAA ACTTTGACAA GGCCACTGAC ATCGTGACAG 1561 GTCTGAAACA GAAAACCTCC TTTGGGAGAC CAATGTGGGA CAATGAGTTT TCTACAATAG 1621 CTACCTCCCA CCCCAAGTCT GTAGTGGGAG TTTTCTTATG TGGCCCTCGG ACTTTGGCAA 1681 AGAGCCTGCG CAAATGCTGT CACCGATATT CCAGTCTGGA TCCTAGAAAG GTTCAATTCT 1741 ACTTCAACAA AGAAAATTTT TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1801 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1861 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATACA CATTGTTGAA 1921 CGCTATTTTC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt