Construct: ORF TRCN0000478067
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017726.1_s317c1
- Derived from:
- ccsbBroadEn_10029
- DNA Barcode:
- CAATTATTCAGCTGGAAGCCAATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RTL4 (340595)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478067
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 340595 | RTL4 | retrotransposon Gag like 4 | NM_001004308.2 | 99.6% | 99.6% | 80T>C;249A>C;657T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 999
- ORF length:
- 930
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagaagtgc acgaaatcat catctaccat gcaggtagag ccttcctttc 121 ttcaggcaga gaatctgatt ctgcggcctc aaatgcagca tccaaccacg gagaacactg 181 ctaaaagggg ccaagtcatg cctgccctgg ccaccacagt gatgcctgta ccatactcac 241 ttgagcatct cacccagttt catggtgacc ctgccaattg ctcagagttc ctcactcagg 301 tgactaccta cttgaccgct ctccaaatct ctaatcctgc aaatgatgcc cagatcaaac 361 tcttttttga ttacctatct cagcagttag aaagttgtgg gatcatatct gggcctgaca 421 agagtacctt actgaagcaa tatgagaatc ttattcttga gttccagcag tcatttggta 481 aacccacaaa acaggaaatc aatcctctga tgaatgctaa gtttgacaaa ggagacaact 541 cctctcagca ggaccctgct actttccacc tcctcgctca aaatctgatc tgtaatgaaa 601 ccaatcagag tggtcagttc gaaaaggcac tagctgatcc caaccaggat gaagagagtg 661 tcactgatat gatggacaat cttccagacc tgatcactca gtgcattcag ttggacaaga 721 aacacagtga caggccagag ctcctacagt cagagaccca gctcccattg ttggcttcct 781 tgatccaaca ccaagccctc tttagcccca cagaTCCACC ACCCAAGAAA GGGCCTATAC 841 AGCTGCGAGA AGGCCAGCTG CCTCTCACCC CAGCCAAACG AGCCCGCCAG CAAGAAACTC 901 AGTTGTGCCT CTACTGCAGC CAATCTGGTC ACTTCACAAG AGATTGCCTT GCCAAACGTT 961 CTCGAGCTCC GGCAACGACA AATAACACAG CTCACCAGTT GCCAACTTTC TTGTACAAAG 1021 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1081 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1141 ACGACAATTA TTCAGCTGGA AGCCAATCAC GCGTTAAGTC gacaatcaac ctctggatta 1201 caaaatttgt gaaagatt