Construct: ORF TRCN0000478075
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006891.1_s317c1
- Derived from:
- ccsbBroadEn_11774
- DNA Barcode:
- GAAAGACCATGCATAGAACGCAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ABTB2 (25841)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478075
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25841 | ABTB2 | ankyrin repeat and BTB doma... | NM_145804.3 | 81.8% | 81.8% | 1_558del |
| 2 | mouse | 99382 | Abtb2 | ankyrin repeat and BTB (POZ... | NM_178890.3 | 73.2% | 79.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2586
- ORF length:
- 2517
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagcgccggc gacgggctgc gccggggcaa gtccgcgcgc tgcggcctca 121 ccttctcagt gggtcgcttt ttccgctgga tggtggacac ccgaatctcc gtgcgcatcc 181 acgagtacgc agccatctcc ctcaccgcct gcatggagaa cctggtggag gagatccggg 241 ccagggtgat ggccagccac agccctgatg gcggaggggc cggaggcggg gaggtgtctg 301 ctgaggccct ggagatggtc atcaacaacg acgccgagct ctggggcgtc ttgcagccct 361 atgagcatct catctgcggc aagaacgcca atggtgtcct ctccctcccc gcatacttca 421 gcccctacaa cggcgggtcc ctgggccatg acgagcgagc cgatgcctat gcccagctgg 481 agctccgaac cctggagcag tccctcctgg ccacctgcgt gggcagcatc tcggagctga 541 gtgacttggt ctcccgtgcc atgcaccaca tgcaggggcg tcaccccctg tgcccgggtg 601 ccagccctgc ccgccaggcc cgccagccgc cacagcccat cacttggtcc cccgacgccc 661 tccacacgct ctactacttt ctgcggtgtc cacagatgga gtccatggag aaccccaacc 721 tggacccccc gagaatgacc ttgaacaatg aacggccctt catgctgctg ccgcccctca 781 tggagtggat gcgcgtggcc atcacctacg cagagcaccg ccgcagcctc accgtggaca 841 gcggcgacat ccggcaggca gcccggctgc tgctgcctgg tctggactgt gaacctcggc 901 agctcaaacc cgaacactgt ttcagttcct tccggaggct ggatgcccga gcagctactg 961 aaaaattcaa ccaggacctg ggtttccgca tgctcaactg tgggaggacg gacctcatca 1021 accaagccat cgaggccttg ggtccggatg gggttaacac catggatgac cagggtatga 1081 cgccactgat gtacgcctgc gctgctgggg acgaagcgat ggtccagatg ttgattgatg 1141 ctggggcaaa cttggacatc caggttccaa gcaactcccc caggcaccct tccatccacc 1201 ccgacagccg gcactggacc tcactgacat tcgctgtgct gcatggacac atctctgtgg 1261 tccagttgct gctggatgct ggtgcccatg tcgagggctc ggcagtgaac ggcggcgagg 1321 acagctatgc ggagacgccc ctgcagctgg cctctgcggc agggaactat gagctggtca 1381 gtttgttgct gagccgaggc gccgaccccc tcctcagcat gctggaggcc cacggcatgg 1441 gctcctccct ccacgaggac atgaactgct tcagccactc agctgcccac ggccacagga 1501 acgtcctgag gaagctcctg acgcagccac agcaggctaa agcggacgtg ctgtccctgg 1561 aggagatcct ggccgagggt gtggaggaaa gtgatgcgtc gagccagggc agtggcagcg 1621 aggggcccgt gcggctgagc cggacccgca ccaaggccct acaggaggcc atgtactaca 1681 gcgctgagca cggctacgtg gacatcacca tggagctgag ggcactggga gtcccctgga 1741 agctgcacat ctggatcgag tctctgagga cctcgttctc gcagtcgcgg tactcggtgg 1801 tgcagagcct cctgcgggac ttcagctcca tcagagagga ggagtacaac gaggagctgg 1861 tgaccgaggg cttgcagctc atgttcgaca tcctcaagac cagcaaaaac gactccgtca 1921 tccagcaact ggctaccatc ttcacccact gctatggcag cagtcccatc cccagcatcc 1981 cagagatccg gaagaccctg ccggccaggc tagatccaca ctttttgaac aataaggaga 2041 tgtcagatgt gaccttcctg gtggaaggaa agctgtttta tgcacataaa gtcctgctgg 2101 tgacagcttc taacaggttc aagacactaa tgaccaataa atcagaacag gatggggaca 2161 gcagcaagac catcgagaTC AGCGACATGA AGTACCACAT TTTTCAGATG ATGATGCAGT 2221 ATCTGTACTA CGGAGGAACA GAATCCATGG AGATCCCCAC CACTGACATC CTGGAGCTGC 2281 TGTCAGCTGC CAGCCTGTTC CAGCTGGATG CCCTGCAGAG GCACTGCGAG ATCCTGTGCT 2341 CCCAGACCCT CAGCATGGAG AGTGCCGTGA ACACCTACAA ATATGCCAAG ATCCACAATG 2401 CCCCAGAACT GGCCCTGTTC TGTGAGGGCT TCTTCCTCAA GCACATGAAG GCCCTACTGG 2461 AGCAGGATGC CTTCCGGCAG CTCATCTACG GCCGCAGCAG CAAAGTGCAG GGCCTGGATC 2521 CACTGCAGGA CCTGCAGAAC ACCCTGGCAG AGCGCGTGCA CTCTGTCTAC ATCACCTCCC 2581 GGGTGTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2641 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2701 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGAAAGACCA TGCATAGAAC GCAAGACGCG 2761 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt