Construct: ORF TRCN0000478078
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015210.1_s317c1
- Derived from:
- ccsbBroadEn_08698
- DNA Barcode:
- AAGAACCGGTCCGCGATAATCGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYSLTR2 (57105)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478078
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57105 | CYSLTR2 | cysteinyl leukotriene recep... | NM_001308465.3 | 99.9% | 99.7% | 475T>A |
| 2 | human | 57105 | CYSLTR2 | cysteinyl leukotriene recep... | NM_001308467.3 | 99.9% | 99.7% | 475T>A |
| 3 | human | 57105 | CYSLTR2 | cysteinyl leukotriene recep... | NM_001308468.3 | 99.9% | 99.7% | 475T>A |
| 4 | human | 57105 | CYSLTR2 | cysteinyl leukotriene recep... | NM_001308469.3 | 99.9% | 99.7% | 475T>A |
| 5 | human | 57105 | CYSLTR2 | cysteinyl leukotriene recep... | NM_001308470.3 | 99.9% | 99.7% | 475T>A |
| 6 | human | 57105 | CYSLTR2 | cysteinyl leukotriene recep... | NM_001308471.3 | 99.9% | 99.7% | 475T>A |
| 7 | human | 57105 | CYSLTR2 | cysteinyl leukotriene recep... | NM_001308476.3 | 99.9% | 99.7% | 475T>A |
| 8 | human | 57105 | CYSLTR2 | cysteinyl leukotriene recep... | NM_020377.5 | 99.9% | 99.7% | 475T>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1107
- ORF length:
- 1038
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagagaaaa tttatgtcct tgcaaccatc catctccgta tcagaaatgg 121 aaccaaatgg caccttcagc aataacaaca gcaggaactg cacaattgaa aacttcaaga 181 gagaattttt cccaattgta tatctgataa tatttttctg gggagtcttg ggaaatgggt 241 tgtccatata tgttttcctg cagccttata agaagtccac atctgtgaac gttttcatgc 301 taaatctggc catttcagat ctcctgttca taagcacgct tcccttcagg gctgactatt 361 atcttagagg ctccaattgg atatttggag acctggcctg caggattatg tcttattcct 421 tgtatgtcaa catgtacagc agtatttatt tcctgaccgt gctgagtgtt gtgcgtttcc 481 tggcaatggt tcaccccttt cggcttctgc atgtcaccag catcaggagt gcctggatcc 541 tcagtgggat catatggatc cttatcatgg cttcctcaat aatgctcctg gacagtggct 601 ctgagcagaa cggcagtgtc acatcatgct tagagctgaa tctctataaa attgctaagc 661 tgcagaccat gaactatatt gccttGGTGG TGGGCTGCCT GCTGCCATTT TTCACACTCA 721 GCATCTGTTA TCTGCTGATC ATTCGGGTTC TGTTAAAAGT GGAGGTCCCA GAATCGGGGC 781 TGCGGGTTTC TCACAGGAAG GCACTGACCA CCATCATCAT CACCTTGATC ATCTTCTTCT 841 TGTGTTTCCT GCCCTATCAC ACACTGAGGA CCGTCCACTT GACGACATGG AAAGTGGGTT 901 TATGCAAAGA CAGACTGCAT AAAGCTTTGG TTATCACACT GGCCTTGGCA GCAGCCAATG 961 CCTGCTTCAA TCCTCTGCTC TATTACTTTG CTGGGGAGAA TTTTAAGGAC AGACTAAAGT 1021 CTGCACTCAG AAAAGGCCAT CCACAGAAGG CAAAGACAAA GTGTGTTTTC CCTGTTAGTG 1081 TGTGGTTGAG AAAGGAAACA AGAGTATTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 1141 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1201 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAAGAACCG 1261 GTCCGCGATA ATCGGTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1321 aagatt