Construct: ORF TRCN0000478119
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001131.1_s317c1
- Derived from:
- ccsbBroadEn_01730
- DNA Barcode:
- GCAATAACTGAACTTGTACAATAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TYRP1 (7306)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478119
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7306 | TYRP1 | tyrosinase related protein 1 | NM_000550.3 | 100% | 100% | |
| 2 | human | 7306 | TYRP1 | tyrosinase related protein 1 | XR_001746372.2 | 51.8% | 1_189del;897_898ins205;1596_2505del | |
| 3 | mouse | 22178 | Tyrp1 | tyrosinase-related protein 1 | NM_001282014.1 | 85.6% | 85.2% | (many diffs) |
| 4 | mouse | 22178 | Tyrp1 | tyrosinase-related protein 1 | NM_001282015.1 | 85.6% | 85.2% | (many diffs) |
| 5 | mouse | 22178 | Tyrp1 | tyrosinase-related protein 1 | NM_031202.3 | 85.6% | 85.2% | (many diffs) |
| 6 | mouse | 22178 | Tyrp1 | tyrosinase-related protein 1 | XM_006537781.1 | 74% | 73.2% | (many diffs) |
| 7 | mouse | 22178 | Tyrp1 | tyrosinase-related protein 1 | XM_006537782.1 | 74% | 73.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1677
- ORF length:
- 1611
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tgctcctaaa ctcctctctc tgggctgtat cttcttcccc ttgctacttt 121 ttcagcaggc ccgggctcaa ttcccaagac agtgtgccac tgttgaggct ttgagaagtg 181 gtatgtgttg cccagacctg tcccctgtgt ctgggcctgg gacagaccgc tgtggctcat 241 catcagggag gggcagatgt gaggcagtga ctgcagactc ccggccccac agccctcagt 301 atccccatga tggcagagat gatcgggagg tctggccctt gcgcttcttc aataggacat 361 gtcactgcaa cggcaatttc tcaggacaca actgtgggac gtgccgtcct ggctggagag 421 gagctgcctg tgaccagagg gttctcatag tcaggagaaa tcttctggac ttaagtaaag 481 aagaaaagaa ccactttgtc cgggccctgg atatggcaaa gcgcacaact caccctttat 541 ttgtcattgc caccaggaga tcagaagaaa tactggggcc agatggcaac acgccacaat 601 ttgagaacat ttccatttat aactactttg tttggacaca ctattactca gtcaaaaaga 661 ctttccttgg ggtaggacag gaaagctttg gtgaagtgga tttctctcat gagggaccag 721 cttttctcac atggcacagg taccacctcc tgcgtctgga gaaagacatg caggaaatgt 781 tgcaagagcc ttctttctcc cttccttact ggaattttgc aacggggaaa aatgtctgtg 841 atatctgcac ggatgacttg atgggatcca gaagcaactt tgattccact ctaataagcc 901 caaactctgt cttttctcaa tggcgagtgg tctgtgactc cttggaagat tatgataccc 961 tgggaacact ttgtaacagc accgaggatg ggccaattag gagaaatcca gctggaaatg 1021 tggccagacc aatggtgcaa cgtcttcctg aaccacagga tgtcgctcag tgcttggaag 1081 ttggtttatt tgacacgcct cctttttatt ccaactctac aaacagtttc cgaaacacag 1141 tggaaggtta cagtgacccc acgggaaagt atgaccctgc tgttcgaagt cttcacaatt 1201 tggctcatct attcctgaat ggaacagggg gacaaaccca tttgtctcca aatgatccta 1261 tttttgtcct cctgcacacc ttcacagatg cagtcttTGA TGAATGGCTG AGGAGATACA 1321 ATGCTGATAT ATCCACATTT CCATTGGAAA ATGCCCCTAT TGGACATAAT AGACAATACA 1381 ACATGGTGCC ATTCTGGCCC CCAGTCACCA ACACAGAAAT GTTTGTTACT GCTCCAGACA 1441 ACCTGGGATA CACTTATGAA ATTCAATGGC CAAGTCGGGA GTTTAGTGTA CCTGAGATAA 1501 TTGCCATAGC AGTAGTTGGC GCTTTGTTAC TGGTTGCACT CATTTTTGGG ACTGCTTCTT 1561 ATCTGATTCG TGCCAGACGC AGTATGGATG AAGCTAACCA GCCTCTCCTC ACTGATCAGT 1621 ATCAATGCTA TGCTGAAGAA TATGAAAAAC TCCAGAATCC TAATCAGTCT GTGGTCTACC 1681 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1741 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1801 ATATATCTTG TGGAAAGGAC GAGCAATAAC TGAACTTGTA CAATAAACGC GTTAAGTCga 1861 caatcaacct ctggattaca aaatttgtga aagatt