Construct: ORF TRCN0000478190
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015505.1_s317c1
- Derived from:
- ccsbBroadEn_01545
- DNA Barcode:
- TTGTTCTTCGAGCGACAGAAAGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC3A2 (6520)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478190
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6520 | SLC3A2 | solute carrier family 3 mem... | NM_001013251.3 | 100% | 100% | |
| 2 | human | 6520 | SLC3A2 | solute carrier family 3 mem... | NM_001012664.3 | 93.1% | 93.1% | 1_117del |
| 3 | human | 6520 | SLC3A2 | solute carrier family 3 mem... | NM_002394.6 | 83.9% | 83.9% | 1_303del |
| 4 | human | 6520 | SLC3A2 | solute carrier family 3 mem... | NM_001012662.3 | 83.8% | 83.8% | 1_306del |
| 5 | human | 6520 | SLC3A2 | solute carrier family 3 mem... | NR_037193.2 | 66.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1653
- ORF length:
- 1587
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ccaggacacc gaggtggata tgaaggaggt ggagctgaat gagttagagc 121 ccgagaagca gccgatgaac gcggcgtctg gggcggccat gtccctggcg ggagccgaga 181 agaatggtct ggtgaagatc aaggtggcgg aagacgaggc ggaggcggca gccgcggcta 241 agttcacggg cctgtccaag gaggagctgc tgaaggtggc aggcagcccc ggctgggtac 301 gcacccgctg ggcactgctg ctgctcttct ggctcggctg gctcggcatg cttgctggtg 361 ccgtggtcat aatcgtgcga gcgccgcgtt gtcgcgagct accggcgcag aagtggtggc 421 acacgggcgc cctctaccgc atcggcgacc ttcaggcctt ccagggccac ggcgcgggca 481 acctggcggg tctgaagggg cgtctcgatt acctgagctc tctgaaggtg aagggccttg 541 tgctgggtcc aattcacaag aaccagaagg atgatgtcgc tcagactgac ttgctgcaga 601 tcgaccccaa ttttggctcc aaggaagatt ttgacagtct cttgcaatcg gctaaaaaaa 661 agagcatccg tgtcattctg gaccttactc ccaactaccg gggtgagaac tcgtggttct 721 ccactcaggt tgacactgtg gccaccaagg tgaaggatgc tctggagttt tggctgcaag 781 ctggcgtgga tgggttccag gttcgggaca tagagaatct gaaggatgca tcctcattct 841 tggctgagtg gcaaaatatc accaagggct tcagtgaaga caggctcttg attgcgggga 901 ctaactcctc cgaccttcag cagatcctga gcctactcga atccaacaaa gacttgctgt 961 tgactagctc atacctgtct gattctggtt ctactgggga gcatacaaaa tccctagtca 1021 cacagtattt gaatgccact ggcaatcgct ggtgcagctg gagtttgtct caggcaaggc 1081 tcctgacttc cttcttgccg gctcaacttc tccgactcta ccagctgatg ctcttcaccc 1141 tgccagggac ccctgttttc agctacgggg atgagattgg cctggatgca gctgcccttc 1201 ctggacagcc tatggaggct ccagtcatgc tgtgggatga gtccagcttc cctgacatcc 1261 caggggctgt aagtgccaac atgactgtga agggccagag tgaagaccct ggctccctcc 1321 tttccttgtt ccggcggctg agtgaccagc ggagtaagga gcgctcccta ctgcatgggg 1381 acttccacgc gttcTCCGCT GGGCCTGGAC TCTTCTCCTA TATCCGCCAC TGGGACCAGA 1441 ATGAGCGTTT TCTGGTAGTG CTTAACTTTG GGGATGTGGG CCTCTCGGCT GGACTGCAGG 1501 CCTCCGACCT GCCTGCCAGC GCCAGCCTGC CAGCCAAGGC TGACCTCCTG CTCAGCACCC 1561 AGCCAGGCCG TGAGGAGGGC TCCCCTCTTG AGCTGGAACG CCTGAAACTG GAGCCTCACG 1621 AAGGGCTGCT GCTCCGCTTC CCCTACGCGG CCTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1681 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1741 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATT 1801 GTTCTTCGAG CGACAGAAAG GGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1861 ttgtgaaaga tt