Construct: ORF TRCN0000478208
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002118.1_s317c1
- Derived from:
- ccsbBroadEn_06783
- DNA Barcode:
- AGGTCAAGAGCCTGGGTCCTGATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LGMN (5641)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478208
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5641 | LGMN | legumain | NM_001008530.3 | 99.7% | 99.7% | 52G>A;720T>C;1251G>A |
| 2 | human | 5641 | LGMN | legumain | NM_005606.7 | 99.7% | 99.7% | 52G>A;720T>C;1251G>A |
| 3 | human | 5641 | LGMN | legumain | XM_017021463.1 | 99.7% | 99.7% | 52G>A;720T>C;1251G>A |
| 4 | human | 5641 | LGMN | legumain | XM_017021464.1 | 99.7% | 99.7% | 52G>A;720T>C;1251G>A |
| 5 | human | 5641 | LGMN | legumain | XM_017021465.1 | 99.7% | 99.7% | 52G>A;720T>C;1251G>A |
| 6 | human | 5641 | LGMN | legumain | XM_011536970.2 | 90.7% | 90.3% | (many diffs) |
| 7 | human | 5641 | LGMN | legumain | NM_001363696.2 | 86.6% | 86.6% | (many diffs) |
| 8 | human | 5641 | LGMN | legumain | XM_005267862.4 | 86.6% | 86.6% | (many diffs) |
| 9 | human | 5641 | LGMN | legumain | NM_001363699.2 | 81.8% | 80.8% | (many diffs) |
| 10 | human | 5641 | LGMN | legumain | XM_005267863.4 | 81.8% | 80.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1365
- ORF length:
- 1299
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ttggaaagta gctgtattcc tcagtgtggc cctgggcatt ggtgccattc 121 ctatagatga tcctgaagat ggaggcaagc actgggtggt gatcgtggca ggttcaaatg 181 gctggtataa ttataggcac caggcagacg cgtgccatgc ctaccagatc attcaccgca 241 atgggattcc tgacgaacag atcgttgtga tgatgtacga tgacattgct tactctgaag 301 acaatcccac tccaggaatt gtgatcaaca ggcccaatgg cacagatgtc tatcagggag 361 tcccgaagga ctacactgga gaggatgtta ccccacaaaa tttccttgct gtgttgagag 421 gcgatgcaga agcagtgaag ggcataggat ccggcaaagt cctgaagagt ggcccccagg 481 atcacgtgtt catttacttc actgaccatg gatctactgg aatactggtt tttcccaatg 541 aagatcttca tgtaaaggac ctgaatgaga ccatccatta catgtacaaa cacaaaatgt 601 accgaaagat ggtgttctac attgaagcct gtgagtctgg gtccatgatg aaccacctgc 661 cggataacat caatgtttat gcaactactg ctgccaaccc cagagagtcg tcctacgcct 721 gttactatga tgagaagagg tccacgtacc tgggggactg gtacagcgtc aactggatgg 781 aagactcgga cgtggaagat ctgactaaag agaccctgca caagcagtac cacctggtaa 841 aatcgcacac caacaccagc cacgtcatgc agtatggaaa caaaacaatc tccaccatga 901 aagtgatgca gtttcagggt atgaaacgca aagccagttc tcccgtcccc ctacctccag 961 tcacacacct tgacctcacc cccagccctg atgtgcctct caccatcatg aaaaggaaac 1021 tgatgaacac caatgatctg gaggagtcca ggcagctcac ggaggagatc cagcggcatc 1081 tggatgccag gcaccTCATT GAGAAGTCAG TGCGTAAGAT CGTCTCCTTG CTGGCAGCGT 1141 CCGAGGCTGA GGTGGAGCAG CTCCTGTCCG AGAGAGCCCC GCTCACGGGG CACAGCTGCT 1201 ACCCAGAGGC CCTGCTGCAC TTCCGGACCC ACTGCTTCAA CTGGCACTCC CCCACGTACG 1261 AGTATGCGTT GAGACATTTG TACGTGCTGG TCAACCTTTG TGAGAAGCCG TATCCACTTC 1321 ACAGGATAAA ATTGTCCATG GACCACGTGT GCCTTGGTCA CTACTGCCCA ACTTTCTTGT 1381 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1441 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1501 GAAAGGACGA AGGTCAAGAG CCTGGGTCCT GATCACGCGT TAAGTCgaca atcaacctct 1561 ggattacaaa atttgtgaaa gatt