Construct: ORF TRCN0000478221
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008879.1_s317c1
- Derived from:
- ccsbBroadEn_01978
- DNA Barcode:
- GCCCACTCGCTACGATAAATGTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCDHGB4 (8641)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478221
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8641 | PCDHGB4 | protocadherin gamma subfami... | NM_032098.1 | 100% | 100% | |
| 2 | human | 8641 | PCDHGB4 | protocadherin gamma subfami... | NM_003736.3 | 86.8% | 86.5% | (many diffs) |
| 3 | human | 56101 | PCDHGB5 | protocadherin gamma subfami... | NM_032099.1 | 82.6% | 81.8% | (many diffs) |
| 4 | human | 56101 | PCDHGB5 | protocadherin gamma subfami... | NM_018925.2 | 73.1% | 72.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2478
- ORF length:
- 2409
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggggagcggc gccggggagc tgggccgggc tgagaggctg ccagtgctct 121 ttctcttcct gctgtctttg ttctgcccgg cgctctgtga gcagatccgc tacaggattc 181 ccgaggaaat gcccaagggc tccgtagtgg ggaacctcgc cacggacctg gggttcagcg 241 tccaggagtt accgactcga aaactgcgcg tcagttcgga gaagccttac ttcaccgtga 301 gcgcagagag cggggagttg cttgtgagca gcaggctaga cagggaggag atatgcggga 361 agaagccagc ttgtgctctg gaatttgagg ctgttgctga aaatccactg aacttttatc 421 acgtgaatgt ggagatcgag gacattaatg accacacgcc aaaattcacg caaaattcct 481 ttgagctgca aataagtgag tctgcacagc ctggcacacg atttatatta ggatctgccc 541 atgatgcgga tattggtagc aacacactgc agaattacca actcagtccc agtgatcatt 601 tctcactgat aaataaagag aaatcagatg gcagtaaata ccctgagatg gtattgaaga 661 cacctttgga cagagaaaag cagaaatcct accacttgac tttgactgcc ttggactttg 721 gagctccacc cctaagcagc actgcacaga tacacgttct agtgactgat gccaatgata 781 atgctccagt gttcagtcaa gacgtataca gggtgagcct ttcagaaaac gtgtacccgg 841 ggaccacggt gctacaggtg actgccacgg accaggatga gggtgtcaat gccgagatta 901 ctttctcttt cagtgaagct agccagatca cccaatttga cctgaactct aacaccgggg 961 aaattactgt tttaaataca ttagattttg aagaagtcaa agaatattcc atagttttgg 1021 aagcaaggga cggtggagga atgattgcgc aatgcacagt ggaggtagaa gtcatagatg 1081 aaaatgacaa cgccccagaa gtgatattcc agtctctacc caacctaatt atggaggacg 1141 ccgagctggg aacacatatt gctttgctca aagtccgtga caaggattcc agacacaatg 1201 gagaagtgac ttgtaaattg gaaggtgatg ttccatttaa aatattaact tcttcaagaa 1261 acacgtataa attagtgaca gatgctgttc tagaccgcga gcagaatcca gagtacaata 1321 taaccgttac ggcaacagat cggggcaagc ctcccctctc ctccagttcc agcatcaccc 1381 tgcacattgg tgatgtaaat gacaacgctc cggttttctc acagtcttcc tatatagtcc 1441 acgtggccga gaacaacccg cctggagcct ctatttcaca agtcagggct tctgatccgg 1501 acttggggcc caacggccaa gtctcttact gcatcatggc cagtgacctg gagcagcggg 1561 agctgtcatc ctacgtgtcc ataagcgcgg agagcggggt ggtgttcgcg cagcgcgcct 1621 tcgaccacga gcagctgcgc gccttcgaac tcacactgca ggcccgcgac cagggctcgc 1681 cagcgctcag cgcgaacgtg agcctgcgcg tgttagtgga cgaccgcaac gacaatgcgc 1741 cacgggtgct gtaccccgcg ctgggtcccg acggctctgc gctcttcgat atggtgccgc 1801 acgctgcaga gcctggctac ttggtgacca aggtagtggc ggtggacgca gactcaggac 1861 acaacgcctg gctgtcctac cacgtgctgc aggctagcga gcccgggctc ttcagcctgg 1921 ggctgcgcac gggcgaagtg cgcacagcgc gtgccttagg cgacagggac gccgtccgcc 1981 agcgccttct ggtcgccgtg cgtgacggtg gacagccacc actctcggcc actgccacgt 2041 tgcacctggt cttcgccgac agcttgcagg aggtgctgcc ggatatcact gaccgccccg 2101 acccctctga cctccaggct gagctgcagt tttacctagt ggtggccttg gccttgatct 2161 cagtgctctt cctcgtggcc atgattctgg ccattgcctt gcgcctgcga cgctcctcca 2221 gccccgcctc ctGGAGCTGC TTCCAGCCTG GTCTCTGTGT TAAATCCGAA TCCGTGGTTC 2281 CCCCCAACTA CAGCGAGGGG ACTTTGCCTT ATTCCTACAA TCTATGTGTT GCACATACAG 2341 GAAAGACGGA GTTTAATTTC CTAAAATGTA GTGAGCAGTT GAGTTCAGGA CAAGACATAC 2401 TTTGCGGTGA TTCATCTGGG GCCTTATTTC CACTTTGTAA TTCCAGTGAA TTGACTTCCC 2461 ATCAGGTGAG TTTCCTTTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2521 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2581 GTATTTCGAT TTCTTGCTTT ATATATCTTG TGGAAAGGAC GAGCCCACTC GCTACGATAA 2641 ATGTGGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt