Construct: ORF TRCN0000478228
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011306.2_s317c1
- Derived from:
- ccsbBroadEn_11589
- DNA Barcode:
- GCTATTCCCCTTTACGTTGGTTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TOPBP1 (11073)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478228
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11073 | TOPBP1 | DNA topoisomerase II bindin... | NM_007027.4 | 94.2% | 94.2% | 1_261del |
| 2 | human | 11073 | TOPBP1 | DNA topoisomerase II bindin... | XM_017005636.2 | 94.2% | 94.2% | 1_261del |
| 3 | human | 11073 | TOPBP1 | DNA topoisomerase II bindin... | NM_001363889.2 | 93.9% | 93.8% | 1_261del;922_923insGTCGTACTCTTTCAG |
| 4 | human | 11073 | TOPBP1 | DNA topoisomerase II bindin... | XR_001739988.2 | 80.3% | 1_359del;3619_3620ins70;4595_5203del | |
| 5 | human | 11073 | TOPBP1 | DNA topoisomerase II bindin... | XM_017005637.2 | 75.7% | 75.7% | 0_1ins1044 |
| 6 | human | 11073 | TOPBP1 | DNA topoisomerase II bindin... | XM_011512357.3 | 68.2% | 68% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4374
- ORF length:
- 4305
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcaccaccag cgatgtgtcc caagagccga acatccagtt tataatatgg 121 ttatgtctga tgtaaccata tcttgtacaa gtctggaaaa agaaaaaagg gaagaagttc 181 ataaatatgt acaaatgatg ggcggacgag tatacagaga ccttaatgta tcagtaactc 241 accttattgc aggagaagtt ggtagcaaaa aatatttagt tgctgcaaac ctgaagaaac 301 ctattttgct tccctcttgg ataaaaacac tttgggagaa gtcacaagag aaaaaaataa 361 ctagatatac tgatataaac atggaagatt tcaagtgtcc tatttttctt ggttgcataa 421 tctgtgtgac tggcttatgt ggcttagaca ggaaagaagt tcagcaactc acagttaagc 481 atggaggtca atacatggga caattgaaaa tgaatgaatg tacacacctc attgtgcaag 541 aaccaaaagg tcagaagtat gagtgtgcca agagatggaa tgtacactgt gtgaccacac 601 agtggttttt tgacagtatt gagaaaggtt tttgtcagga tgaatccata tacaagacag 661 aacctagacc agaagcaaag actatgccca attcttcaac tcctaccagc cagatcaaca 721 caattgatag tcgtactctt tcagatgtca gcaatatttc caacataaat gcaagttgcg 781 taagtgaatc aatatgtaat tcacttaaca gcaaactgga gcctacactt gaaaatctag 841 aaaatctgga tgtcagtgca tttcaagcac ctgaagattt attagatggt tgtcggatat 901 atctttgcgg ttttagtggc agaaagctag ataaactgag aagacttatt aacagtggag 961 gtggagttcg ttttaaccag ctaaatgaag atgtaactca tgttattgtg ggagattatg 1021 atgatgaatt gaagcagttt tggaataaat cagcccacag gcctcatgta gtgggagcaa 1081 agtggttgct agagtgtttc agtaaaggtt atatgctttc tgaagaacca tatatccatg 1141 ctaattacca gccagtggaa attccagttt cacataagcc tgaaagtaaa gcagctcttt 1201 taaaaaagaa gaacagcagc ttctctaaga aagactttgc tcctagtgaa aagcatgagc 1261 aagctgatga agatctgctc tctcaatatg aaaatggtag ctccacagta gttgaggcta 1321 agacgtctga agccaggccc tttaatgatt ctactcatgc tgagcccttg aatgattcta 1381 ctcacatttc tttgcaagaa gaaaaccagt cttctgtcag tcattgtgtc cctgatgttt 1441 ctacaattac tgaagaaggc ttatttagcc aaaagagttt ccttgttttg ggttttagta 1501 atgaaaatga atctaacatc gcaaacatca taaaagaaaa tgctgggaaa atcatgtccc 1561 ttctgagcag aactgttgcg gattatgctg tggttcctct gctggggtgt gaagtggaag 1621 ccactgtggg agaagttgtt acaaatacat ggctggttac ttgcatagac tatcagactt 1681 tgtttgatcc aaagtcgaat cctctcttca caccagttcc agtaatgaca ggaatgactc 1741 ctttagagga ttgtgttatt tcatttagcc agtgtgctgg agcagaaaaa gagtctttaa 1801 cattcctagc aaacctcctt ggagcaagtg ttcaagaata ctttgttcgc aaatccaatg 1861 caaagaaagg catgtttgcc agtactcatc ttatactgaa agaacgtggt ggctctaaat 1921 atgaagctgc aaagaagtgg aatttacctg ccgttactat agcttggctg ttggagactg 1981 ctagaacggg aaagagagca gacgaaagcc attttctgat tgaaaattca actaaagaag 2041 aacgaagttt ggaaacagaa ataacaaatg gaatcaatct aaattcagat actgcagagc 2101 atcctggcac acgcctgcaa actcacagaa aaaccgtcgt tacaccttta gatatgaacc 2161 gctttcagag taaagctttc cgtgctgtgg tctcacaaca tgccagacag gtcgcagcct 2221 ccccagcagt aggacaacca cttcagaagg agccctcgtt acacctggat acaccatcaa 2281 aattcctgtc caaggacaaa ctcttcaagc cttcctttga tgtgaaggat gcacttgcag 2341 ccttggaaac tccaggacgt cccagccaac agaaaaggaa accgagtacg ccactctcag 2401 aagttattgt caaaaacttg caacttgctt tggcaaatag ctctcgaaat gctgtcgctc 2461 tttctgccag ccctcaactg aaagaggccc agtcagagaa ggaagaagcc ccaaagccac 2521 ttcacaaagt agtggtatgt gttagtaaaa aactcagtaa gaagcagagt gaactaaatg 2581 ggatcgcagc ctctctagga gcagattaca ggtggagttt tgatgaaaca gtgactcatt 2641 tcatctatca agggcggcca aatgacacta atcgggagta taaatctgta aaagaaagag 2701 gagtacacat tgtttccgag cactggcttt tagattgtgc ccaagagtgt aaacatcttc 2761 ctgaatctct ttatccacat acttataatc ccaaaatgag cttggatatc agcgcagtgc 2821 aagatggccg gctctgtaat agtcgactac tctcagctgt gtcttcaaca aaggatgatg 2881 agccagatcc tttgatttta gaagaaaatg atgtagacaa tatggccacc aataataaag 2941 agtcagcacc atcaaatgga agtggaaaga atgactctaa aggagttctg acacagacct 3001 tagagatgag agagaacttt cagaagcagt tacaggagat aatgtctgca acatcaatag 3061 tgaaacccca agggcagagg acttcccttt caagaagtgg ttgtaacagc gcatcttcaa 3121 cccctgacag cactcgctct gctcgcagtg gacgaagtag agtcctagag gcactgaggc 3181 agtctcgtca gacagtacct gatgtcaaca cagagccttc ccaaaatgaa cagatcattt 3241 gggatgaccc tacagcaagg gaggagagag caaggcttgc cagcaatttg cagtggccta 3301 gttgtcccac acaatactct gagcttcagg ttgacattca aaacttggag gattctcctt 3361 ttcaaaagcc tttacatgat tcagaaattg ctaaacaggc tgtctgtgat cctggaaaca 3421 tacgtgtgac tgaagctccc aaacacccaa tctctgaaga actggaaact cccataaaag 3481 acagccacct gatccctacg cctcaagccc ccagtattgc ctttccactc gccaaccccc 3541 ctgtggctcc gcaccctaga gaaaagatta taacgataga ggagactcat gaagaattaa 3601 aaaaacagta catatttcag ttatcatctc tgaatcctca agaacgtatt gactattgtc 3661 atctgattga gaaactaggt ggattggtga tagaaaagca gtgctttgat cccacctgta 3721 cacacattgt tgtgggacat ccacttcgaa acgagaagta tttagcctca gtggcagctg 3781 ggaagtgggt gcttcatcgc tcctaccttg aagcctgcag gactgctgga cacttcgtgc 3841 aggaagaaga ctatgaatgg ggaagtagtt ccatacttga tgttctgact ggaatcaatg 3901 tacagcaacg aagactagca cttgcagcaa tgagatggag aaaaaaaatc cagcaaagac 3961 aagaatctgg cattgttgag ggagcattta gtgggtggaa ggttatttta catgtggatc 4021 agtctcgaga agcaggcttc aaacgccttc ttcagtcagg aggagcaaag gtgctacctg 4081 gtcattctgt acctttattt aaagaggcca cacaTCTTTT TTCTGACTTG AATAAACTGA 4141 AACCAGATGA CTCAGGAGTT AATATAGCAG AAGCTGCTGC CCAGAACGTG TACTGCTTGA 4201 GAACAGAATA CATTGCTGAT TATCTCATGC AGGAATCACC TCCTCATGTA GAAAATTACT 4261 GTCTACCAGA AGCTATTTCA TTTATTCAGA ATAATAAGGA ACTTGGGACT GGATTATCAC 4321 AAAAGAGGAA AGCTCCTACA GAAAAAAATA AAATCAAACG ACCTAGAGTA CACTTGCCAA 4381 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 4441 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 4501 TATCTTGTGG AAAGGACGAG CTATTCCCCT TTACGTTGGT TAAACGCGTT AAGTCgacaa 4561 tcaacctctg gattacaaaa tttgtgaaag att