Construct: ORF TRCN0000478255
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016456.1_s317c1
- Derived from:
- ccsbBroadEn_05425
- DNA Barcode:
- ACTTGGCAGCTGTTAGTTGCCTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNAI3 (333929)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478255
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 333929 | SNAI3 | snail family transcriptiona... | NM_178310.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 945
- ORF length:
- 876
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccgcgctcc ttcctggtga aaacgcactc cagccacagg gtccccaact 121 accggcggct ggagacgcag agagaaatca atggtgcctg ctctgcctgt ggggggctgg 181 tggtgcccct cctcccccga gacaaggagg ccccttctgt gcccggtgac cttccccagc 241 cctgggaccg ctcctcggcc gtcgcctgca tctccctgcc cctcctgcca cggatcgagg 301 aagctctggg ggcctctggg ctggacgcct tggaagtcag cgaggtcgac cctcgggcca 361 gccgggccgc cattgtaccc ctcaaagaca gcctgaacca cctcaacctg cccccactgc 421 tggtgctgcc cacacggtgg tccccgacct tgggcccaga ccggcacggg gctccggaaa 481 aactgcttgg ggctgagcgg atgccccgag ccccgggcgg ctttgagtgc ttccactgcc 541 acaaacccta ccacacgctg gccgggctgg ccaggcaccg gcagctgcac tgccacctgc 601 aggtggggcg tgtcTTCACC TGCAAGTACT GCGACAAGGA GTACACCAGC CTGGGTGCCC 661 TCAAGATGCA CATCCGCACT CACACGCTGC CCTGCACCTG CAAGATCTGT GGCAAGGCCT 721 TCTCCAGGCC CTGGTTACTG CAGGGCCATG TCCGCACCCA CACAGGGGAG AAGCCCTATG 781 CCTGCTCGCA CTGCAGCAGG GCCTTTGCCG ACCGCTCCAA CCTTCGGGCC CATCTGCAAA 841 CGCACTCAGA CGCCAAGAAG TACCGGTGCC GGCGCTGCAC CAAGACCTTC TCCCGCATGT 901 CCCTCCTGGC GCGGCATGAG GAGTCTGGCT GCTGCCCGGG CCCCTTGCCA ACTTTCTTGT 961 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1021 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1081 GAAAGGACGA ACTTGGCAGC TGTTAGTTGC CTTTACGCGT TAAGTCgaca atcaacctct 1141 ggattacaaa atttgtgaaa gatt