Construct: ORF TRCN0000478309
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010507.1_s317c1
- Derived from:
- ccsbBroadEn_04872
- DNA Barcode:
- ACCGAATCCGACGTTTTCATGCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LYZL4 (131375)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478309
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 131375 | LYZL4 | lysozyme like 4 | NM_001304386.2 | 100% | 100% | |
| 2 | human | 131375 | LYZL4 | lysozyme like 4 | NM_144634.4 | 100% | 100% | |
| 3 | human | 131375 | LYZL4 | lysozyme like 4 | XM_017005706.1 | 100% | 100% | |
| 4 | human | 131375 | LYZL4 | lysozyme like 4 | XM_024453345.1 | 100% | 100% | |
| 5 | human | 131375 | LYZL4 | lysozyme like 4 | XM_011533355.3 | 88% | 83.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 504
- ORF length:
- 438
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ggcatccgtg gttctctccc tccttggcta cctggtggtt ccaagtggtg 121 cttacatctt ggggcgttgc acagtggcta agaaactcca cgatggaggc ctggattatt 181 ttgagggcta tagccttgag aactgggtgt gcctggccTA CTTCGAGAGC AAGTTCAACC 241 CCATGGCCAT CTACGAGAAC ACACGTGAGG GCTACACTGG CTTTGGCCTC TTTCAGATGC 301 GTGGCAGTGA CTGGTGTGGC GACCATGGCA GGAACCGCTG CCATATGTCA TGTTCCGCTT 361 TACTGAATCC TAATTTAGAG AAGACAATTA AATGTGCCAA GACCATTGTA AAAGGAAAAG 421 AAGGGATGGG AGCATGGCCC ACCTGGTCCC GGTACTGCCA GTACTCCGAT ACCCTGGCAC 481 GGTGGCTGGA TGGTTGCAAG CTGTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 541 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 601 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA CCGAATCCGA 661 CGTTTTCATG CTTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 721 att