Construct: ORF TRCN0000478314
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011240.1_s317c1
- Derived from:
- ccsbBroadEn_00246
- DNA Barcode:
- CGTTCCCGTATCATATAGTTCAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CD247 (919)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478314
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 919 | CD247 | CD247 molecule | NM_198053.2 | 100% | 100% | |
| 2 | human | 919 | CD247 | CD247 molecule | NM_000734.4 | 99.3% | 99.3% | 299_300insGCA |
| 3 | human | 919 | CD247 | CD247 molecule | XM_011510144.2 | 89.2% | 85.2% | (many diffs) |
| 4 | human | 919 | CD247 | CD247 molecule | XM_011510145.1 | 88.6% | 84.6% | (many diffs) |
| 5 | human | 919 | CD247 | CD247 molecule | XM_017002800.1 | 84.1% | 84.1% | 58_150del |
| 6 | human | 919 | CD247 | CD247 molecule | XM_017002801.1 | 83.5% | 83.5% | 58_150del;392_393insGCA |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 558
- ORF length:
- 492
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagaag 61 ttggcatgaa gtggaaggcg cttttcaccg cggccatcct gcaggcacag ttgccgatta 121 cagaggcaca gagctttggc ctgctggatc ccaaactctg ctacctgctg gatggaatcc 181 tcttcatcta tggtgtcatt ctcactgcct tgttcctgag agtgaagttc agcaggagcg 241 cagacgcccc cgcgtaccag cagggccaga accagctcta taacgagctc aatctaggac 301 gaagagagga gtacgatgtt ttggacaaga gacgtggccg ggacccTGAG ATGGGGGGAA 361 AGCCGCAGAG AAGGAAGAAC CCTCAGGAAG GCCTGTACAA TGAACTGCAG AAAGATAAGA 421 TGGCGGAGGC CTACAGTGAG ATTGGGATGA AAGGCGAGCG CCGGAGGGGC AAGGGGCACG 481 ATGGCCTTTA CCAGGGTCTC AGTACAGCCA CCAAGGACAC CTACGACGCC CTTCACATGC 541 AGGCCCTGCC CCCTCGCTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 601 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 661 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACGTTCCC GTATCATATA 721 GTTCAATACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt