Construct: ORF TRCN0000478318
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014068.1_s317c1
- Derived from:
- ccsbBroadEn_07765
- DNA Barcode:
- CACACACGCGCCATATTGATCTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- INMT (11185)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478318
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11185 | INMT | indolethylamine N-methyltra... | NM_006774.5 | 99.6% | 98.8% | 82G>A;613A>G;761T>G |
| 2 | human | 11185 | INMT | indolethylamine N-methyltra... | NM_001199219.2 | 99.2% | 98.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 858
- ORF length:
- 789
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagggtggc ttcactgggg gtgatgagta ccagaagcac ttcctgccca 121 gggactactt ggctacttac tacagcttca atggcagccc ctcacccgag gccgagatgc 181 tgaagtttaa cttggaatgt ctccacaaga ccttcggccc tggaggcctc caaggggaca 241 cgctgattga cattggctca ggtcctacca tctaccaagt tcttgctgcc tgtgattcct 301 tccaagacat cactctctcc gactttaccg accgcaaccg ggaggagctg gaaaagtggc 361 tgaagaagga gccgggggcc tatgactgga ccccagcggt gaaattcgcc tgtgagctgg 421 aaggaaacag cggccgatgg gaggagaagg aggagaagct gcgggcagcg gtgaagcggg 481 tgctcaagtg cgatgtccac ctgggcaacc cgctggcccc ggctgtgttg cctctcgccg 541 actgtgtgct caccctgctg gccatgGAGT GTGCCTGCTG TAGCCTTGAT GCCTACCGCG 601 CTGCCCTGTG CAACCTTGCC TCACTGCTCA AGCCGGGTGG CCACCTGGTG ACCACTGTCA 661 CGCTTCGGCT CCCGTCCTAC GTGGTGGGGA AGCGTGAATT TTCCTGCGTG GCCCTGGAGA 721 AAGAGGAGGT GGAGCAGGCT GTCCTGGATG CTGGCTTTGA CATTGAACAG CTCCTACACA 781 GTCCCCAGAG CTACTCTGTC ACCAATGCTG CCAACAATGG GGTCTGCTGC ATTGTGGCTC 841 GCAAGAAGCC TGGGCCCTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 901 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 961 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACACACAC GCGCCATATT 1021 GATCTTTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt