Construct: ORF TRCN0000478339
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018420.2_s317c1
- Derived from:
- ccsbBroadEn_05485
- DNA Barcode:
- GGCATACGGCTCAGGAGGTCCGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF677 (342926)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478339
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 342926 | ZNF677 | zinc finger protein 677 | NM_001317998.1 | 100% | 100% | |
| 2 | human | 342926 | ZNF677 | zinc finger protein 677 | NM_182609.3 | 100% | 100% | |
| 3 | human | 342926 | ZNF677 | zinc finger protein 677 | XM_011526912.2 | 100% | 100% | |
| 4 | human | 342926 | ZNF677 | zinc finger protein 677 | XM_005258862.4 | 90.1% | 91% | 0_1ins142;28_61del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1818
- ORF length:
- 1752
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tctttctcag ggactgttta cattcaagga tgtggccata gaattctctc 121 aagaggagtg ggagtgcctg gaccctgccc agagggcctt gtacagggac gtgatgttgg 181 agaactacag gaacctgctt tctctcgatg aggataacat ccctccagaa gatgatattt 241 ctgttggatt tacaagcaag ggattatcac caaaggaaaa taataaagag gaattatacc 301 atctggtgat attagaaaga aaggaaagcc atggcatcaa caattttgac ctcaaggaag 361 tctgggaaaa tatgcctaag tttgacagcc tgtgggacta tgatgtaaaa aattacaaag 421 gaatgccttt gacctgtaac aaaaatctca ctcacagaaa agatcaacaa cataataaat 481 cctcaataca tttctcttta aagcagagtg tttctataag agatagtgca caccagtatt 541 tcatccatga caagccattt ataaggaatt tgttaaaact gaaaaataac ataaggtatg 601 ccggaaacaa atacgtgaag tgttttgaaa ataaaattgg attaagctta caggcacagc 661 tggctgaact acagagattt caaactgggg agaaaatgta tgaatgtaat ccagttgaga 721 agtctatcaa tagttcctca gtttcaccac ttcctccttg tgtcaaaaac atttgtaata 781 aatataggaa gattttgaaa taccctttat tacatacaca gtatgggaga acacacatta 841 gagaaaaatc atacaagtgt aatgactgtg gaaaggcttt tagcaaaagt tcgaacctca 901 ctaatcatca gagaattcac tctggacaga gaccttacaa atgtaacgag tgtggcaaag 961 cctttaacca gtgttcgaac ctcactaggc atcagagagt ccatacagga gagaaaccat 1021 atcaatgtaa tatatgtggc aaggtctgta gtcaaaattc aaatcttgca agtcatcaga 1081 ggatgcatac tggagagaaa ccttacaaat gtaatgaatg tggtaaggca tttatccagc 1141 gttcacacct ttggggtcat gaaagaattc atactggaga gaaaccttac aaatgtaatg 1201 aatgtgacaa agcctttgct gaacgttcaa gccttaccca acataagaga atccatactg 1261 gagagaagcc ttacatatgt aatgagtgtg gcaaagcttt taagcagtgc tcacatctca 1321 ctaggcatca gaatatacat cctggagaga aaccacacaa atgtaatgtg tgtggcaggg 1381 cttttatcca aagttcaagt cttgtggaac atcagagaat tcacactgga gaaaaacctt 1441 acaaatgtaa taaatgtgat aaagctttta tcaaacgttc acacctttgg ggtcatcaga 1501 gaactcatac tggagagaaa CCTTACAAAT GTACTGAATG TGGCAAAGCC TTTACTGAAC 1561 GTTCAAATCT TACTCAGCAT AAGAAAATCC ATACTGGAGA GAAACCTTAC AAATGTACTG 1621 AATGTGGCAA AGCTTTTACC CAATTTGCAA ACCTCACTAG ACACCAGAAA ATACACATTG 1681 AAAAGAAACA TTGTAAACAT AATATACATG GTAATGCTTT ATTCCAAAGT TCAAACCTTG 1741 GAGATCATCA AAAAAGTTAT AATAGAGAAA AACATATCAA ATATAATGAG ACAAAAATTA 1801 AGTATTCAAG CTGTACCTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1861 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1921 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGGCATAC GGCTCAGGAG 1981 GTCCGGTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt