Construct: ORF TRCN0000478358
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009473.1_s317c1
- Derived from:
- ccsbBroadEn_07258
- DNA Barcode:
- TACCATCTGGAAGTTTTACAGCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- APOL1 (8542)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478358
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8542 | APOL1 | apolipoprotein L1 | NM_001136540.1 | 99.5% | 99.2% | (many diffs) |
| 2 | human | 8542 | APOL1 | apolipoprotein L1 | NM_003661.4 | 99.5% | 99.2% | (many diffs) |
| 3 | human | 8542 | APOL1 | apolipoprotein L1 | NM_145343.2 | 95.7% | 95.4% | (many diffs) |
| 4 | human | 8542 | APOL1 | apolipoprotein L1 | NM_001136541.1 | 95% | 94.7% | (many diffs) |
| 5 | human | 8542 | APOL1 | apolipoprotein L1 | NM_001362927.2 | 95% | 94.7% | (many diffs) |
| 6 | human | 8542 | APOL1 | apolipoprotein L1 | XM_011530478.2 | 69.1% | 68.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1263
- ORF length:
- 1194
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggnnncat ggagggagct gctttgctga gagtctctgt cctctgcatc tggatgagtg 121 cacttttcct tggtgtggga gtgagggcag aggaagctgg agcgagggtg caacaaaacg 181 ttccaagtgg gacagatact ggagatcctc aaagtaagcc cctcggtgac tgggctgctg 241 gcaccatgga cccagagagc agtatcttta ttgaggatgc cattaagtat ttcaaggaaa 301 aagtgagcac acagaatctg ctactcctgc tgactgataa tgaggcctgg aacggattcg 361 tggctgctgc tgaactgccc aggaatgagg cagatgagct ccgtaaagct ctggacaacc 421 ttgcaagaca aatgatcatg aaagacaaaa actggcacga taaaggccag cagtacagaa 481 actggtttct gaaagagttt cctcggttga aaagtnagct tgaggataac ataagaaggc 541 tccgtgccct tgcagatggg gttcagaagg tccacaaagg caccaccatc gccaatgtgg 601 tgtctggctc tctcagcatt tcctctggca tcctgaccct cgtcggcatg ggtctggcac 661 ccttcacaga gggaggcagc cttgtactct tggaacctgg gatggagttg ggaatcacag 721 cagctttgac cgggattacc agcagtacca tagactacgg aaagaagtgg tggacacaag 781 cccaagccca cgacctggtc atcaaaagcc ttgacaaatt gaaggaggtg aaggagtttt 841 tgggtgagaa catatccaac tttctttcct tagctggcaa tacttaccaa ctcacacgag 901 gcattgggaa ggacatccgt gccctcagac gagccagagc caatcttcag tcagtaccgc 961 atgccTCAGC CTCACGCCCC CGGGTCACTG AGCCAATCTC AGCTGAAAGC GGTGAACAGG 1021 TGGAGAGAGT TAATGAACCC AGCATCCTGG AAATGAGCAG AGGAGTCAAG CTCACGGATG 1081 TGGCCCCTGT AAGCTTCTTT CTTGTGCTGG ATGTAGTCTA CCTCGTGTAC GAATCAAAGC 1141 ACTTACATGA GGGGGCAAAG TCAGAGACAG CTGAGGAGCT GAAGAAGGTG GCTCAGGAGC 1201 TGGAGGAGAA GCTAAACATT CTCAACAATA ATTATAAGAT TCTGCAGGCG GACCAAGAAC 1261 TGTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1321 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1381 GGCTTTATAT ATCTTGTGGA AAGGACGATA CCATCTGGAA GTTTTACAGC GAACGCGTTA 1441 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt