Construct: ORF TRCN0000478386
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017890.2_s317c1
- Derived from:
- ccsbBroadEn_03363
- DNA Barcode:
- TTGCAATATTGAAAAATTATCTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TPPP3 (51673)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478386
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51673 | TPPP3 | tubulin polymerization prom... | NM_015964.4 | 100% | 100% | |
| 2 | human | 51673 | TPPP3 | tubulin polymerization prom... | NM_016140.4 | 100% | 100% | |
| 3 | human | 51673 | TPPP3 | tubulin polymerization prom... | XM_024450294.1 | 100% | 100% | |
| 4 | mouse | 67971 | Tppp3 | tubulin polymerization-prom... | NM_026481.3 | 89.7% | 96.5% | (many diffs) |
| 5 | mouse | 67971 | Tppp3 | tubulin polymerization-prom... | XM_006531324.3 | 89.7% | 96.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 594
- ORF length:
- 528
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agcgagcaca gacatggctg ggctggagga gagcttccgc aagtttgcca 121 tccatggtga ccccaaggcc agtgggcaag agatgaatgg caagaactgg gccaagctgt 181 gcaaggactg caaggtggct gacggaaagt ccgtgacagg gaccgatgtg gacatcgtct 241 tctccaaagt caaggggaag tctgctcggg tcaTCAACTA TGAGGAGTTC AAGAAGGCCC 301 TGGAAGAGCT GGCGACCAAG AGATTCAAGG GGAAGAGCAA GGAGGAGGCC TTCGATGCCA 361 TCTGCCAGCT GGTGGCAGGC AAAGAGCCAG CCAATGTGGG CGTCACTAAA GCAAAAACAG 421 GGGGTGCTGT AGACCGGCTG ACGGACACCA GCAGATACAC GGGCTCCCAC AAGGAGCGCT 481 TCGATGAGAG CGGCAAGGGC AAGGGCATTG CGGGACGGCA GGACATCCTG GACGACAGTG 541 GCTACGTGAG CGCCTACAAG AATGCAGGCA CCTACGATGC CAAGGTGAAG AAGTGCCCAA 601 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 661 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 721 TATCTTGTGG AAAGGACGAT TGCAATATTG AAAAATTATC TTTACGCGTT AAGTCgacaa 781 tcaacctctg gattacaaaa tttgtgaaag att