Construct: ORF TRCN0000478394
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000816.2_s317c1
- Derived from:
- ccsbBroadEn_12523
- DNA Barcode:
- ACTCAGAGCCTAATCTGCCATATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OTUB2 (78990)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478394
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 78990 | OTUB2 | OTU deubiquitinase, ubiquit... | NM_023112.4 | 31.1% | 31.1% | 220_702del |
| 2 | mouse | 68149 | Otub2 | OTU domain, ubiquitin aldeh... | NM_026580.4 | 29% | 29.4% | (many diffs) |
| 3 | mouse | 68149 | Otub2 | OTU domain, ubiquitin aldeh... | XM_006516185.3 | 24.4% | 24.5% | (many diffs) |
| 4 | mouse | 68149 | Otub2 | OTU domain, ubiquitin aldeh... | NM_001177841.1 | 21% | 21% | (many diffs) |
| 5 | mouse | 68149 | Otub2 | OTU domain, ubiquitin aldeh... | NR_033587.1 | 6.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 309
- ORF length:
- 219
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGAGTGAAAC ATCTTTCAAC CTAATATCAG 121 AAAAATGTGA CATTCTATCC ATTCTTCGGG ACCATCCTGA AAACAGGATT TACCGGAGGA 181 AAATCGAGGA ACTCAGCAAA AGGTTCACCG CCATCCGCAA GACCAAAGGG GATGGGAACT 241 GCTTCTACAG GGCCTTGGGC TATTCCTACC TGGAGTCCCT GCTGGGGAAG AGCAGGGAGA 301 TCTTCAAGTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 361 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 421 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAACTCAG AGCCTAATCT GCCATATGAC 481 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt