Construct: ORF TRCN0000478402
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010382.2_s317c1
- Derived from:
- ccsbBroadEn_14392
- DNA Barcode:
- CCGTAAACAACGTTCGAAAATATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WDR66 (144406)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478402
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 144406 | WDR66 | WD repeat domain 66 | NM_144668.6 | 99.4% | 99.2% | (many diffs) |
| 2 | human | 144406 | WDR66 | WD repeat domain 66 | NM_001178003.2 | 80.7% | 79.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3528
- ORF length:
- 3462
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc agatgcagca gaagctcccc gagaagcaac aggagaaaat ggagaaacag 121 aaatgaaaga agaggaggaa cctaatccaa attataaaga agtagaagat ccacaacagg 181 aatcaaaaga tgacacaata gcatggagag agtctcagga ggaggagagg aaaacgggcg 241 aggaggaagg ggaggaggag gagaaagagg aggaggggaa ggaggacaaa aagattgtca 301 tggaagaaac tgaggaaaag gctggagaag tccaagagaa ggaggcttca ggaatacagg 361 aagaaaccac agtagagccc caagaagtca cagcgtccat gatccgtttg gagacacaga 421 ttactgattc ccagtcaatc acatcaggaa ttttcccaaa aacccaaaga ggtagcaagt 481 caaagctttc cttacaattg gaggatgcag aaacagatga gcttttaaga gacctgagca 541 cacaaattga atttcttgat ttggatcaaa tcagtcctga ggaacaacag attagttccc 601 ctgaaaggca gccctcagga gagcttgagg agaaaaccga ccggatgccc caagatgaac 661 tgggacaaga aagaagggac ttggagccag aaaacagaga ggagggacaa gaaaggagag 721 tatccgacat ccagtccaaa gcagggatct cccgggagtc actggtgtcc agcaccacag 781 aggacattct gtttcaaaag gataaaagca ccccggtgta tcccttgacc atgacctggt 841 cgtttggatg gaacagttct cttcctgttt actatattcg agaggaaagg cagagagttc 901 ttctgtatgt ttgtgctcac actgcgatca tctacaatgt gttcaggaac aatcaatacc 961 accttcaggg ccacgccaat attatctcct gcctctgcgt cagtgaagac aggcggtgga 1021 tcgccacagc agacaaaggg ccagactgcc tggtgattat atgggactcc ttcacaggta 1081 ttcctgtgca cacaatattt gacagctgcc ctgaagggaa tggcatcatg gccatggcca 1141 tgacccacga cgccaagtat ctggcaacca tctcagatgc tgaagtccag aaggtatgca 1201 tctggaagtg gactttggca gtggaaacgc cagcatgcac tctcgaactc cccacagagt 1261 acggtgttca gaactacgtt acttttaacc caacaaataa taaagaattg gtgagcaata 1321 gtaaaacacg ggcaatatat tatgcatggt atgaagagag ggatacactg gctcacagtg 1381 ccccactttt aactgaaaaa accttcaaca agcttgtggg aaagtttagc cagtccatct 1441 ttcacttgaa tttaacacaa atactctcag ccacaatgga agggaagctg gttgtctggg 1501 acatacaccg cccaccctca tctgcctcca cctttttggg ctttccctat atcaagcctt 1561 gtaaattggt tcatttgcag aaagagggta tcacggtact taccacaatt gatagctaca 1621 ttgtcacagg tgacattaag gggaacatta agttctatga tcacaccctg tctattgtta 1681 actggtacag tcacttgaaa ctgggcgcca taagaactct gtccttttca aagaccccag 1741 caactcctcc tactgaaaaa tcaaactatc ctcctgactg cactttaaaa ggtgaccttt 1801 ttgtcttaag gaattttatc attggaacat ctgatgccgc ggtgtaccac ttaacaacag 1861 atgggaccaa acttgagaag ttatttgtag agcccaagga tgccatttgt gccatctcct 1921 gccacccata tcaacccctc attgccatcg ggagcatctg tgggatgatc aaagtgtgga 1981 attatgaaaa caaacaatat cttttcagca gggtttttga gaaggggttt ggagtccaga 2041 gtctgaccta caaccccgaa ggagcccttc ttggagctgg ctttacagag gggacagttt 2101 acattcttga tgcaatgtct ttagaaaatg aaagcccaga gcctttcaaa tattccagaa 2161 ccagtgtgac tcatataagc ttttcccatg actcccagta tatggcaact gctgatagaa 2221 gttttactgt ggctgtttac atgctggtgg tcagaaatgg acagagggtc tgggagtact 2281 tagcaagact tcgctctcat cgcaaaagca ttcgaagtct cctgtttggg gtttacctgg 2341 acagcaatga gcctagactg ctgagccttg ggacagacag gctcttgata gagtatgatc 2401 ttctcaggag ctacaaagac cacctggaag tcctggacat tcaccacacc gaccagggct 2461 gctatcccac ctgcatggtc tggtacccac cactcaccag ggaactcttc ctgcttattt 2521 gcaacagtgg ctacaaagtg aagcttttta atgctactac caaaatgtgc agaaagacgc 2581 ttctggggcc agcttatggt tcccctattg agcagacaca agtcctccca gtgagaagca 2641 tggcggagct acagaaacgc tacttggtgt ttattaacag agacaagttg ggacttcaga 2701 tcttaccagt tgacggcaat ccacataaga catctgctat tgtttgccac ccgaacgggg 2761 tggccggcat ggccgtttcc tatgatggct gctacgcctt cactgcggga gggcacgatc 2821 gctcggtggt gcagtggaaa atcaccttaa gtgtcctgga ggcagcggtt tctcttgggg 2881 gtgaagactt gaccccattc tatggtctgc tgtctggtgg ccgggaagga aaattctaca 2941 gggagctaga agactacttc tactattctc agctccgcag tcaaggcatc gacacaatgg 3001 agaccagaaa ggtgtcagaa cacatttgcc tgtcagagct tccttttgtc atgagagcaa 3061 ttggctttta cccatctgaa gagaagattg atgatatatt taacgaaatc aaatttggtg 3121 aatatgtgga cactggaaag ctaatcgaca agatcaactt accagatttc ctaaaagtgt 3181 accttaacca caagccacct tttggtaaca ccatgagtgg catccacaag agcttTGAGG 3241 TGCTCGGTTA TACCAACTCC AAAGGGAAAA AGGCCATTCG AAGAGAGGAC TTCCTGAGAC 3301 TGCTCGTTAC TAAAGGTGAG CATATGACGG AGGAGGAGAT GTTGGATTGC TTTGCTTCAC 3361 TGTTTGGCCT GAATCCCGAG GGATGGAAAT CCGAGCCTGC AACCTGCTCC GTCAAAGGTT 3421 CAGAAATTTG CCTTGAAGAA GAACTTCCAG ACGAAATCAC TGCAGAAATA TTCGCGACTG 3481 AAATTCTTGG CTTAACCATT TCAGAAGATT CCGCCCAGGA TTGTCAGTGT CCAACTTTCT 3541 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 3601 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 3661 GTGGAAAGGA CGACCGTAAA CAACGTTCGA AAATATTACG CGTTAAGTCg acaatcaacc 3721 tctggattac aaaatttgtg aaagatt