Construct: ORF TRCN0000478403
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010087.1_s317c1
- Derived from:
- ccsbBroadEn_03454
- DNA Barcode:
- GCACTAAATTGCTTTCTCATGACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LEPROT (54741)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478403
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54741 | LEPROT | leptin receptor overlapping... | NM_017526.5 | 100% | 100% | |
| 2 | human | 54741 | LEPROT | leptin receptor overlapping... | NM_001198681.1 | 92.3% | 90.7% | (many diffs) |
| 3 | human | 54741 | LEPROT | leptin receptor overlapping... | NM_001198683.2 | 27.2% | 25.1% | (many diffs) |
| 4 | mouse | 230514 | Leprot | leptin receptor overlapping... | NM_175036.4 | 89% | 94.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 459
- ORF length:
- 393
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gggcgttaaa gctctcgtgg cattatcctt cagtggggct attggactga 121 cttttcttat gctgggatgt gccttagagg attatggcgt ttactggccc ttattcgtcc 181 TGATTTTCCA CGCCATCTCC CCCATCCCCC ATTTCATTGC CAAAAGAGTC ACCTATGACT 241 CAGATGCAAC CAGTAGTGCC TGTCGGGAAC TGGCATATTT CTTCACTACT GGAATTGTTG 301 TTTCTGCCTT TGGATTTCCT GTTATTCTTG CTCGTGTGGC TGTGATCAAA TGGGGAGCCT 361 GCGGCCTTGT GTTGGCAGGC AATGCAGTCA TTTTCCTTAC AATTCAAGGG TTTTTCCTTA 421 TATTTGGAAG AGGAGATGAT TTTAGCTGGG AGCAGTGGTA CCCAACTTTC TTGTACAAAG 481 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 541 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 601 ACGAGCACTA AATTGCTTTC TCATGACAAC GCGTTAAGTC gacaatcaac ctctggatta 661 caaaatttgt gaaagatt