Transcript: Human NM_017526.5

Homo sapiens leptin receptor overlapping transcript (LEPROT), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
LEPROT (54741)
Length:
4541
CDS:
58..453

Additional Resources:

NCBI RefSeq record:
NM_017526.5
NBCI Gene record:
LEPROT (54741)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_017526.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000344840 GCAAATAGACCTGTCAAATTT pLKO_005 625 3UTR 100% 15.000 21.000 N LEPROT n/a
2 TRCN0000061654 CCTTAGAGGATTATGGCGTTT pLKO.1 134 CDS 100% 4.050 5.670 N LEPROT n/a
3 TRCN0000061655 TGGCCCTTATTCGTCCTGATT pLKO.1 157 CDS 100% 4.950 3.960 N LEPROT n/a
4 TRCN0000363686 TGGCCCTTATTCGTCCTGATT pLKO_005 157 CDS 100% 4.950 3.960 N LEPROT n/a
5 TRCN0000425267 AGACCAAGAGCCTCAACATTT pLKO_005 884 3UTR 100% 13.200 9.240 N LEPROT n/a
6 TRCN0000061653 GCCTTTGGATTTCCTGTTATT pLKO.1 298 CDS 100% 13.200 9.240 N LEPROT n/a
7 TRCN0000363746 GCCTTTGGATTTCCTGTTATT pLKO_005 298 CDS 100% 13.200 9.240 N LEPROT n/a
8 TRCN0000434102 GGTAGCACTTTATTCTGATTA pLKO_005 449 CDS 100% 13.200 9.240 N LEPROT n/a
9 TRCN0000353146 TGTCGGGAACTGGCATATTTC pLKO_005 253 CDS 100% 13.200 9.240 N LEPROT n/a
10 TRCN0000061656 CTTATATTTGGAAGAGGAGAT pLKO.1 409 CDS 100% 4.050 2.835 N LEPROT n/a
11 TRCN0000061657 TCACTACTGGAATTGTTGTTT pLKO.1 275 CDS 100% 5.625 3.375 N LEPROT n/a
12 TRCN0000333534 TCACTACTGGAATTGTTGTTT pLKO_005 275 CDS 100% 5.625 3.375 N LEPROT n/a
13 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 4158 3UTR 100% 5.625 2.813 Y KLHL30 n/a
14 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 4158 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_017526.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03454 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_03454 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000478403 GCACTAAATTGCTTTCTCATGACA pLX_317 69.9% 100% 100% V5 n/a
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