Construct: ORF TRCN0000478404

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009545.2_s317c1
Derived from:
ccsbBroadEn_11749
DNA Barcode:
ATCGGCATAACCCGATTTCTCATA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PSD4 (23550)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478404
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23550 PSD4 pleckstrin and Sec7 domain ... XM_006712392.2 97.2% 97.1% (many diffs)
2 human 23550 PSD4 pleckstrin and Sec7 domain ... NM_012455.3 97.1% 97% (many diffs)
3 human 23550 PSD4 pleckstrin and Sec7 domain ... XM_005263634.2 97.1% 97% (many diffs)
4 human 23550 PSD4 pleckstrin and Sec7 domain ... XM_006712394.2 88.6% 77.8% (many diffs)
5 human 23550 PSD4 pleckstrin and Sec7 domain ... XM_006712393.2 88.5% 77.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3147
ORF length:
3081
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgat gggtgactac agactccctg accaccccca gcccatggaa attctcaacc
121tgtacttggg agacagcctg gagccccacc caggagagtg cccaagggaa acgtgcagcc
181atgaggatcc accggagcct ttcgaggagc aaacctgggc cactgaccct cctgaaccta
241ccagacaaaa tgttcctccc tggggctccg gtgtggagct cacacacctg gggagctggg
301tccatcagga cgggctggag ccttgccagg agcaaacccg ggccactgac cctcctgaat
361ctaccagaca agatgctcct ccctggggct ccggtgtgga gctcacacac ctggggagcc
421cctctgccca gagggatcac aggcagaaca cagcatcacc agggtcacca gtgaacagcc
481atctaccggg gagcccaaag cagaaccgga gcacgtccac acaggtagtg ttctgggcag
541gcatcctgca ggcccagatg tgtgtcctag acctggagga ggagctggag aagacggaag
601ggctcaaggc tgggctgaaa tgctgtctcc ccacgccccc tgtggacctc cccggggaca
661cgggcctgca ctccagccca cctgagaatg aagactcagg ggaagacagc agcgagcctg
721agggagaggg ccaggcatgg ctgagagagg gaaccccaga ctcttcccca cagtggggag
781ctgaggagga gagcatgttc ttcagcaacc ccctcttcct ggcgagtcct tgctcagaga
841acagtgcttc tggagagtgc ttttcctggg cggcttcaga ctcccatgca ggtgtgagga
901ctggacctga gagcccagcg actctggagc ctcccctccc agaagacaca gtgctgtggg
961agctggaaag tgagccagat ttgggggacg gcgctgctat cagtgggcat tgtacccctc
1021cattccctgt gcccatctat aaaccacact ccatctgctg ggcctcagtg gctgccgctg
1081agggggctcc tgcagcacct cctggtcacg gggagagtga gggagatagg cttggtcctg
1141ctccatctgc agcaccgtgt gtggacgaag cattgacctg ggaatcagga tgtgtcggat
1201ctgatcttgg ccctgctgca catcctgtgc aaccttgggc ctctctcagc cctgagggct
1261ggcagagagg aggtcctttt tggccccagg tgactcttaa ctcccaggac agagagccta
1321gcagcccaga atctgagagc agaggccctg gtcccaggcc cagccctgca tcgtcccagg
1381agggcagccc gcagcttcaa caccacagct caggcatttt gcccaagtgg acactagatg
1441cttcacagtc ttcactcttg gagacggatg gggaacagcc aagttccttg aagaaaaagg
1501aggcagggga ggccccaaaa ccaggcgagg aagtaaagag tgaaggaaca gccaggcctg
1561cagagactgg agacgtccag cctgacattc acctgacttc tgcagaacat gagaatctga
1621ggacaccgat gaactcttct tggcttcctg ggagccctat gccccaagca cagtccccag
1681aggaaggcca gagaccacca gctggagaca agctagctaa tggcgtcagg aacaacaagg
1741tagcctggaa cttggcctca cgcctctatc gcctggaggg cttccggaag tctgaagtgg
1801ctgcctacct gcagaagaac aatgacttta gcagggctgt ggctgaggag tacctgtcct
1861tcttccagtt tggaggccag agtctggacc gagccctccg gagcttcctc caggccttgg
1921tgctcagtgg ggagactcag gaacgggagc gaatcctcta ccagttctcc agacgcttcc
1981accattgcaa tccggggatc ttcccctcag tagattctgt acacaccttg acatgtgcaa
2041tcatgctgct taacacggac ctgcatggac agaacattgg gaagagcatg agctgccagg
2101aattcataac caacctgaat gggctgaggg atggcgggaa cttccccaag gagctgctga
2161aggccctcta ctggtctatc cgcagcgaga agctcgagtg ggccgtggat gaagaagaca
2221cagccagacc tgagaaggcc cagccgtccc tgccagctgg caagatgagc aagcccttcc
2281ttcagctggc tcaggatccc acagtgccca cctacaagca gggcatcctg gctcggaaaa
2341tgcatcaaga tgcagacggc aagaagacgc catggggcaa gcgtggctgg aagatgttcc
2401acaccttact gcgagggatg gttctctact tcctgaaggg agaagaccac tgtctggagg
2461gggagagctt ggtggggcag atggtggatg agcccgtggg ggtgcaccac tcgctggcca
2521cccccgccac gcattacacc aagaagccgc acgtcttcca gctgcgcacg gctgactggc
2581gcctctacct cttccaggca cccactgcca aggagatgag ctcctggatc gcgcgcatca
2641acttggctgc ggccacccac tccgcgccgc ccttccccgc cgctgtgggc tcccagcgca
2701gattcgtgcg gcccatcctg cccgtgggcc ccgcccagag ctccctggag gagcagcatc
2761gatcccacga gaactgcctg gacgctgccg cggacgacct gctggatcta cagaggaacc
2821tgccggagcg gcggggccgt ggccgcgagc tggaggagca ccgcctgcgg aaggagtacc
2881tggagtacga gaaaacccgc tacgagaccT ACGTGCAGCT GCTGGTGGCC CGCCTGCACT
2941GCCCCTCTGA TGCTCTGGAC CTGTGGGAGG AGCAGCTGGG GAGGGAAGCT GGAGGCACTC
3001GGGAGCCCAA GCTCAGCCTG AAGAAGTCCC ACTCGAGCCC GTCCCTGCAC CAGGATGAGG
3061CTCCCACCAC GGCCAAGGTG AAGCGCAACA TCTCAGAGCG CAGAACCTAC CGGAAGATCA
3121TCCCTAAGCG GAACCGCAAT CAGCTGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG
3181GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
3241AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAATCGGCAT
3301AACCCGATTT CTCATAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
3361aagatt

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